重组葡激酶基因在大肠杆菌内的高水平表达及生物学活性研究  

High-level expression of recombinant staphylokinase in Escherichia coli

作　　者：李倩[1] 荫俊[1] 宋伟[1] 雷祚荣[1] 付玲[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《生物技术通讯》1999年第4期266-269,共4页Letters in Biotechnology

摘　　要：将测序后的葡激酶重组质粒ＰＵＣ－ＳＡＫ经酶切后，组装于表达载体ｐＢＶ２２０，构建成ｐＢＶ－ＳＡＫ表达质粒，转化大肠杆菌。重组葡激酶表达水平达６０％～７０％，相对分子质量为 １５ ５００，主要以可溶状态存在于细胞中。生物活性测定证实，重组葡激酶具有很强的纤溶活性。The recombinant expression plasmid pBV-SAK was constructed by inserting the cDNA fragment of staphylokinase into pBV220 and transformed into E. coli DH5α cell. Induction of transfected E. coli DH5α cell in a bacterial fermentor produced intracellular recombinant staphy- lokinase representing 60%-70% of total cell protein and in soluble form. Staphylokinase activity was measured by using the fibrin plate method.

关 键 词：葡激酶 基因表达 生物活性 

分 类 号：Q786[生物学—分子生物学]