P27基因联合中药片仔癀对人骨肉瘤抑制作用观察  

Inhibitory effect of human P27KIP1 gene AVV virus combining with Chinese herb Pien Tze Huang on human osteosarcoma transplant mice model

作　　者：张俐[1] 曾启清[1] 林建华[2] 

机构地区：[1]福建中医学院,福州350108 [2]福建医科大学附属第一医院,福州350005

出　　处：《中华中医药杂志》2009年第4期511-515,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：卫生部科学研究基金(No.WKJ2005-2-014);福建省高等学校新世纪优秀人才支持计划闽教科(No.[2006]35);卫生部科学研究基金(No.WKJ2008-2-42)

摘　　要：目的:观察P27基因重组腺病毒联合中药片仔癀对人骨肉瘤裸鼠移植瘤体内生长的抑制作用。方法:①用人骨肉瘤细胞株MG-63接种于裸鼠腋部皮下,连续传代3次,肿瘤生长稳定后,再用组织移植法构建人MG-63细胞裸鼠原位骨肉瘤模型;②肿瘤细胞接种1周肿瘤长至0.7cm左右时,选择40只肿瘤体积均一的裸鼠随机分成5组:空白对照组(8只),PBS溶液注射治疗;P27基因治疗组(8只),raav-P27病毒液注射治疗;Paav-MCS空载体组(8只),raav-MCS病毒注射治疗;片仔癀组(8只),片仔癀溶液灌胃治疗;联合治疗组(8只),raav-P27病毒液注射联合片仔癀灌胃治疗。③观测指标:骨肉瘤裸鼠成瘤率、荷瘤裸鼠生活质量及毒副反应、肿瘤块的体积和肿瘤的生长抑制率。结果:①成功构建了裸鼠原位骨肉瘤模型;②单纯灌服片仔癀溶液、P27基因治疗及片仔癀、P27基因联合治疗组肿瘤的生长明显受到抑制,与空白对照组及空载体组差异显著(P<0.05),肿瘤生长抑制率分别达32.24%,55.25%与61.84%。空白对照组与空载体组相比,无显著差异(P>0.05);③荷瘤裸鼠一般情况良好,活动能力较强。结论:①腺相关病毒介导的p27基因能有效抑制人骨肉瘤MG-63的生长。②P72基因与片仔癀联合应用后,产生协同作用,其抑瘤效果与改善荷瘤裸鼠的生存情况比单用片仔癀治疗或单用P27基因治疗更明显。③中药片仔癀对提高荷瘤裸鼠的生活质量有一定的帮助。Objectives： To study the e ect of human P27KIP1 gene AVV virus combining with Chinese herb Pien Tze Huang on human osteosarcoma mice model. Methods： 40 standardized human osteosarcoma mice model were divided into 5 groups, treated with PBS, Paav-MCS, human P27KIP1 gene AVV virus, Chinese herb Pien Tze Huang or human P27KIP1 gene AVV virus combining with Chinese herb Pien Tze Huang in each di erent group. Results： ① Mice model of transplant human osteosarcoma were established successfully. ②Osteosarcoma were inhibited successfully by human P27KIP1 gene AVV virus, Chinese herb Pien Tze Huang or human P27KIP1 gene AVV virus combining with Chinese herb Pien Tze Huang. ③Mice model of transplant human osteosarcoma were active during observation. Conclusions： ①These results suggested that human P27KIP1 gene AVV virus, herb Pien Tze Huang and human P27KIP1 gene AVV virus combined with herb Pien Tze Huang were e ective on human osteosarcoma. ②Among them, the most e ective treatment was human P27KIP1 gene AVV virus combining with herb Pien Tze Huang. ③Chinese herb Pien Tze Huang was possible useful for life improvement of osteosarcoma patient.

关 键 词：P27基因 片仔癀 移植瘤 人骨肉瘤 裸鼠原位骨肉瘤模型 

分 类 号：R738.1[医药卫生—肿瘤]