多发性肌炎湿热证模型的制作  

Establishing model of polymyositis with damp-heat syndrome

作　　者：赖名慧[1] 刘友章[2] 

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院,广州510405

出　　处：《中华中医药杂志》2009年第4期522-525,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广东省科技计划项目(No.2008B060600065)

摘　　要：目的:研究制作实验性多发性肌炎湿热证模型的方法。方法:将45只豚鼠随机分为正常组、对照组(即实验性肌炎组)与湿热组,每组15只。对照组利用家兔骨骼肌匀浆加弗氏完全佐剂为免疫原皮下注射豚鼠,湿热组除上述多次免疫豚鼠外,并结合湿热证造模(肥甘饮食、湿热环境及病原微生物等复合因素)的方法,形成多发性肌炎湿热证模型。观察各组动物其在一般情况、血清肌酶、光镜、电镜方面的改变。结果:发现湿热组在临床表现、血清肌酶及骨骼肌、肺、肝组织的病理改变与人类湿热型的多发性肌炎相近。结论:提示该模型可作为研究人类多发性肌炎湿热证的一个手段,为人类肌炎中医证型的发病机理及治疗提供理论依据。Objective： To research the method of Establishing model of polymyositis with damp-heat syndrome. Methods： 45 guinea pigs were randomly divided into three groups, group A （normal group）, group B （experimental myositis group, control group）, Group C （damp-heat group）. In the control group, the rabbit skeletal muscle homogenate Garver,s complete adjuvant for subcutaneous immunization was used to injecting into the original guinea pigs. Besides the above-mentioned immune method, the heat-damp model method was used （feigan diet, hot and humid environment and pathogenic micro-organisms and other complex factors） to establish the polymyositis model with damp-heat syndrome. The general situations, such as the serum muscle enzymes, light microscopy, electron microscopy changes in each group were observed. Results： The results showed that the clinical performance, serum creatase, the pathological changes of skeletal muscle, lungs, liver of guinea pigs in damp-heat group were similar to the human polymyositis with damp-heat syndrome. Conclusion： The model can be used to study the human polymyositis with damp-heat syndrome, and provide the pathogenic and theoretical basis for treating polymyositis by TCM.

关 键 词：多发性肌炎 动物模型 湿热证 豚鼠 实验研究 

分 类 号：R-332[医药卫生]