新生鼠缺氧缺血性脑病动物模型的建立  

The Establishment of an Experimental Model of Neonatal Hypoxic-ischemic Encephalipathy in Rat

作　　者：胡志兵[1] 郑德枢[2] 陆雪芬[2] 邓平[2] 陈听安[2] 

机构地区：[1]广州市第十二人民医院神经内科,广州510620 [2]广州医学院神经科学研究所,广州510182

出　　处：《广州医学院学报》1999年第3期13-15,共3页Academic Journal of Guangzhou Medical College

摘　　要：目的：建立新生鼠缺氧缺血性脑病动物模型，研究其病理变化。方法：结扎新生７天ＳＤ大鼠一侧颈总动脉后，置于８％氧浓度的低氧环境中２．５ｈ，于缺氧缺血后３ｈ、６ｈ、１ｄ、３ｄ、７ｄ。１４ｄ、２１ｄ取脑做ＨＥ．ＮＩＳＳＬ染色，观察脑组织病理变化。结果：发现缺氧缺血后３ｈ缺血侧出现轻度脑损伤，１ｄ病变最严重，３ｄ出现胶质细胞增生，１４ｄ、２１ｄ出现脑萎缩。结论：本动物模型稳定性良好，其病理变化具有一定的规律性。Objective:To establish an animal model of neonatal hypoxic-ischemic encephalipathy (HIE) and to study the pathological changes in it. Method: 7-day-old rats were subject to left common carotid artery ligation, then were exposed to 8% O2 /92% N_2, for up to 2.5h. At 3h, 6h , 1d, 3d, 7d, 14d, and 21d after the hypoxic-ischemic episode, the rats were killed and the brain tissues were sampled out for typical pathological processing and hematoxylin-eosin (HE) and Nissl (cresyl violet) staining, and the pathological changes of the brain tissues were observed. Result: three hours after the hypoxic-ischemic episode mild local necrosis Of brain tissues began to occur, and the pathological changes became most severe at 1d. Reactive gliosis began at 3d and brain atrophy was observed at 14d or 21d. Conclusion: The model of HIE is stable and the pathological changes of the brain tissues are with certain regularity.

关 键 词：新生鼠 缺氧缺血性脑病 动物模型 病理变化 

分 类 号：R-332[医药卫生]