鼻良恶性病变P_(53)基因表达及DNA倍体相关性研究  

A Study of Correlation Between P_(53) Gene Expression and DNA Ploidy in Benign and Malignant Nasal Diseases

作　　者：张志钢[1] 

机构地区：[1]广州医学院附属市二人民医院耳鼻咽喉科,广州510150

出　　处：《广州医学院学报》1999年第2期16-18,共3页Academic Journal of Guangzhou Medical College

摘　　要：目的：研究Ｐ_（５３）基在在鼻良恶性病变的表达。方法：应用流式细胞术和细胞免疫荧光染色技术，对鼻炎性增生组织１７例、乳头状瘤９例和鼻鳞癌组织３５例的Ｐ_（５３）基因蛋白表达进行定量研究，结果显示：１鼻良性病变 Ｐ_（５３）表达量明显低于鼻鳞癌； ２．Ｐ_（５３）表达阳性率和表达量与鼻鳞癌病理组织学分级密切相关；３．Ｐ_（５３）表达量与鼻鳞癌 ＤＮＡ倍体相关，非整倍体肿瘤 Ｐ_（５３）表达量高于二倍体肿瘤。结论：Ｐ_（５３）基因蛋白表达能区分鼻良恶性肿瘤及鼻恶性肿瘤病理组织学分级，同时还与ＤＮＡ倍体相关。Objectives: To study the expression of P_(53) gene in tissues of benign and malignant nasal dis- eases. Methods: The protein expressions of P_(53) gene were quantitatively analyzed by using flow cy- tometry and cellular immunofluorescence staining technique, in tissue samples from 17 cases of na- sal inflammatory proliferation, 9 cases of nasal papilloma, 35 cases of nasal squamous cancer and 10 cases of normal nasal mucosa tissues. Results: (1)The expression level of P_(53) gene in benign nasal disease tissues was lower than that in nasal squamous cancer tissues. (2) The rate of positive ex- pression of P_(53) and the expression quantity were correlated to the histopathologic grading of nasal squamous cancer. (3) The quantity of P_(53) protein expression was associated with DNA ploidy in nasal squamous cancer; that in aneuploid tumors was higher than that in diploid ones. Conclusion: P_(53) protein expression might be hopeful to become a parameter for distinguishing benign from ma- lignant nasal tumors, and the histopathologic grading of nasal squamous cancer is probably correlat- ed to the DNA ploidy of the cancer cells.

关 键 词：鼻鳞癌 鼻良性病变 DNA倍体 流式细胞术 P₅₃单克隆抗体 

分 类 号：R739.62[医药卫生—肿瘤]