Baculovirus-mediated gene transfer into mammalian cells  

作　　者：朱应 齐义鹏 刘子夜 肖庚富 

出　　处：《Science China(Life Sciences)》1998年第5期473-478,共6页中国科学（生命科学英文版）

摘　　要：A kind of cytotoxinic gene barnase was cloned downstream of immediate early promoter from cytomegalovirus (CMV). Recombinant virus vAcCMVBar was constructed using Bac\|to\|Bac system by insertion of the expression cassette into the polyhedrin gene locus of Autographa californica nuclear polyhedrosis virus (AcNPV). Cytotoxinic effect caused by expression of barnase was observed in four mammalian cells after vAcCMVBar infection. Dose\|dependent analysis revealed that one of the cells HICAM was the most sensitive to the virus infection. It was shown that AcNPV could be used as gene transfer vector in mammalian cells and that barnase is the suitable report gene which could be detected more easily than that commonly used.A kind of cytotoxinic gene barnase was cloned downstream of immediate early promoter from cytomegalovirus (CMV). Recombinant virus vAcCMVBar was constructed using Bac\|to\|Bac system by insertion of the expression cassette into the polyhedrin gene locus of Autographa californica nuclear polyhedrosis virus (AcNPV). Cytotoxinic effect caused by expression of barnase was observed in four mammalian cells after vAcCMVBar infection. Dose\|dependent analysis revealed that one of the cells HICAM was the most sensitive to the virus infection. It was shown that AcNPV could be used as gene transfer vector in mammalian cells and that barnase is the suitable report gene which could be detected more easily than that commonly used.

关 键 词：ACNPV BARNASE gene MAMMALIAN cells. 

分 类 号：Q789[生物学—分子生物学] Q959.8