氧化砷对胃癌细胞的细胞毒和诱导凋亡作用  

The Cytotoxicity and Induction of Apoptosis by Arsenic Trioxide on Gastric Cancer Cell SGC-7901

作　　者：江石湖[1] 涂水平[1] 谭继宏[1] 蒋晓华[1] 乔敏敏[1] 章永平[1] 吴云林[1] 吴裕■ 

机构地区：[1]上海第二医科大学附属瑞金医院消化内科,200025

出　　处：《胃肠病学》1998年第4期228-231,共4页Chinese Journal of Gastroenterology

基　　金：上海市科委自然科学发展基金(No.9641219035)

摘　　要：目的:研究三氧二化砷(氧化砷)对胃癌细胞的细胞毒和诱导凋亡作用。方法:应用MTT、TUNEL染色、流式细胞仪技术研究氧化砷对不同分化程度的胃癌细胞的细胞毒和诱导凋亡的作用。结果:氧化砷对不同分化程度的胃癌细胞均具有较强的细胞毒作用.10μmol/L氧化砷作用24小时细胞杀伤率为24.6％ 48小时接近50％,氧化砷作用于不同分化程度的胃癌细胞后,可看到较为典型的细胞凋亡的形态学变化:细胞核固缩,染色质疑集.呈新月型紧贴于核膜周边.核碎裂.染色质片断化,凋亡小体形成等,流式细胞仪DNA直方图上出现典型的亚二倍体的“凋亡峰”TU-NEL染色法显示,细胞凋亡指数为1.4％～9.5％。氧化砷的细胞毒作用呈时间和剂量依赖性,且表现为细胞周期特异性,作用48小时后,SGC-7901细胞的细胞周期变化明显,G0/G1期细胞从54.2％下降到17.7％ 而G2/M期细胞则从20.2％上升到63.4％,说明氧化砷主要作用于细胞周期的G2/M期,抑制细胞增殖和诱导细胞凋亡。结论:氧化砷对胃癌细胞具有较强的细胞毒和诱导细胞凋亡的作用.具有治疗胃癌的潜在价值。Background/Aims: To study the effects of arsenic trioxide (As2O3) on gastric cancer cells in cytotoxicity and induction of apoptosis. Methodes: The cytotoxicity of As2O on gastric cancer cells was determined by MTT assay. Apoptosis and cell cycle changes of SGC-7901 induced by As2O were investigated by TUNEL method and flow cytometry. Results: As2O had a remarkablely cytotoxic effect on differnt differentiated gastric cancer cells. The cytotoxic rate of 10μmol/L As2O3 on gastric cancer cells approximates 50.0% in 48 hours. After 12 hours of exposure to the drug, SGC-7901 exhibited morphologic features of apoptosis. including cell shrinkage, nuclear condensation. DNA fragmentation and formation of apoptotic bodies. A typical subdiploid peak before GO/ G1 phase was observed by flow cytometry. The apoptotic index was 1.4%-9.5% by TUNEL. The effect of As2O on SGC-7901 showed a remarkable cell cycle specificity, which induced death in G1 phase, and blocked at G2/M phase. Conclusions: As2O3 exhibites significantly the cytotoxicity on gastric cancer cells and induction of apoptosis. As2O3 might have a promising prospect in the treatment of gastric cancer, and was worthy to be further studied.

关 键 词：胃癌细胞 氧化砷 细胞凋亡 细胞毒 

分 类 号：R735.2[医药卫生—肿瘤]