生防放线菌153活菌剂填料的选择及其防病促生作用研究  

Formulation of biocontrol actinomyces strain 153 and its control effect on Verticillium wilt

作　　者：骞天佑[1] 唐彩乐[1] 田晓丽[1] 宗兆锋[1] 

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2009年第4期169-172,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：教育部创新团队发展计划项目(200558);西北农林科技大学创新团队项目

摘　　要：【目的】筛选153活体菌剂的理想填料,以延长153制剂的货架期,提高其大田防病效果。【方法】以固体发酵培养153,分别添加硅藻土、高岭土和非耕作层黄土3种填料,通过活菌量测定和温室防病促生效果观察对理想填料进行筛选。【结果】在3种填料中,以加入非耕作层黄土的活体粉剂的存储时间最长,35℃下可以保存70 d以上,活菌量保持水平最高。菌株153对茄子的生长发育有明显促进作用,对植株株高、倒3叶叶面积、干质量及根生长的促进作用最为明显,植株鲜质量的增幅最高可达98.47%,地上部干质量和根干质量的增幅最高分别可达74.86%和82.64%。以非耕作层黄土为填料的菌剂对茄子黄萎病的防效可达82.44%。【结论】通过加入合适的填料,能有效延长菌剂贮存期并提高菌株的生态适应性,为生防菌153从实验室走向生产实践奠定了基础。[Objective] The study was done to prolong the shelf life of actinomycetes strain 153 and improve the bio-control effect in the field grown condition. [Method] 153 solid-state fermented culture was mixed with diatomite,kaolin as well as non-cultivated loess and screened by the number of living bacteria and their protection effect on eggplant against Verticillium dahliae was studied. [Result] Non-cultivated loess was better than diatomite,kaolin. With this additive,the biocontrol agent could be stored more than 10 weeks at 35 ℃. The growth ahd development of eggplant could be promoted obviously by live-cell formulation of 153. Especially,the amplitude of green plant weight attained 98.47% ,amplitude of dry weight of stem and dry weight of radix attained 74.86% and 82.64%. The control rate of eggplant Verticillium wilt with the formulation mixed non-cultivated loess reached 82.44 %. [Conclusion] By adding suitable additive, the formulation of aetinomyces strain 153 can obtain longer shelf life and better environment adaptation.

关 键 词：放线菌 生防菌制剂 菌株153 固态发酵 大丽轮枝菌 

分 类 号：S482.39[农业科学—农药学] S154.383[农业科学—植物保护]