Formation of secondary plasmodesmata and intercellular passages by means of ACHT  

作　　者：吴伯骥 谢明唐 崔亚亚 陈毅平 郭卫红 蒋辉 郑国锠 

机构地区：[1]Chengdu Institute of Bioiogy, Chinese Academy of Sciences, Chengdu 610041, China [2]Laboratory of Cell Biology, Lanzhou University, Lanzhou 730000, China

出　　处：《Science China Chemistry》1995年第2期187-194,共8页中国科学（化学英文版）

基　　金：Project supported by the National 863 High Technology Programme Commitee and the Chairman Foundation of the Chinese Academy of Sciences.

摘　　要：By artificially forcing cytoplasm and chromatin migration between different plant cells and through coculture. the isolation layer with uneven Ihickness was formed between tobacco and spinach cells. Then it was gradually absorbed, thinned, and eliminated finally, so that different plant cells were linked together with each other into chimaera. Under further co-culture condition, secondary half-plasmodesmata, secondary plasmodesmata and intercellular passages (cytomictic channels) were formed on one side or both sides of the walls of the adjoining cells, thus different plant cells were linked into symplastic cell mass. This provides the cell structural and cell physiological basis for further manipulating the intercellular migration of cytoplasm and chvomatin. Various intercellular contacts and passages are described.By artificially forcing cytoplasm and chromatin migration between different plant cells and through coculture. the isolation layer with uneven Ihickness was formed between tobacco and spinach cells. Then it was gradually absorbed, thinned, and eliminated finally, so that different plant cells were linked together with each other into chimaera. Under further co-culture condition, secondary half-plasmodesmata, secondary plasmodesmata and intercellular passages (cytomictic channels) were formed on one side or both sides of the walls of the adjoining cells, thus different plant cells were linked into symplastic cell mass. This provides the cell structural and cell physiological basis for further manipulating the intercellular migration of cytoplasm and chvomatin. Various intercellular contacts and passages are described.

关 键 词：ACHT(Artificial Cvtomixis HYBRIDIZATION Technique) CO-CULTURE plasmodesniata cytomictie channel. 

分 类 号：Q25[生物学—细胞生物学]