Nucleotide sequence of maize dwarf mosaic virus capsid protein gene and its expression in Escherichia coli  

作　　者：赛吉庆 康良仪 黄忠 史春霖 田波 谢友菊 

机构地区：[1]Beijing Agricultural University, Beijing 100094, China [2]Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China

出　　处：《Science China Chemistry》1995年第3期313-319,共7页中国科学（化学英文版）

摘　　要：The 3’-terminal 1 279 nucleotide sequence of maize dwarf mosaic virus (MDMV) genome has been determined. This sequence contains an open reading frame of 1023 nudeotides and a 3’ -non-coding region of 256 nucleotides. The open reading frame includes all of the coding regions for the viral capsid protein (CP) and part of the viral nuclear inclusion protein (Nib). The predicted viral CP consists of 313 amino acid residues with a calculated molecular weight of 35400. The amino acid sequence of the viral CP derived from MDMV cDNA shows about 47%-54% homology to that of 4 other potyviruses. The viral CP gene was constructed in frame with the lacZ gene in pUC19 plasmid and expressed in E. coli cells. The fusion polypeptide positively reacted in Western blot with an antiserum prepared against the native viral CP.The 3'-terminal 1 279 nucleotide sequence of maize dwarf mosaic virus (MDMV) genome has been determined. This sequence contains an open reading frame of 1023 nudeotides and a 3' -non-coding region of 256 nucleotides. The open reading frame includes all of the coding regions for the viral capsid protein (CP) and part of the viral nuclear inclusion protein (Nib). The predicted viral CP consists of 313 amino acid residues with a calculated molecular weight of 35400. The amino acid sequence of the viral CP derived from MDMV cDNA shows about 47%-54% homology to that of 4 other potyviruses. The viral CP gene was constructed in frame with the lacZ gene in pUC19 plasmid and expressed in E. coli cells. The fusion polypeptide positively reacted in Western blot with an antiserum prepared against the native viral CP.

关 键 词：MAIZE DWARF MOSAIC virus (MDMV) CAPSID protein (CP) sequence Western blot. 

分 类 号：Q78[生物学—分子生物学]