ER-Ⅱ期乳腺浸润性导管癌的差异表达蛋白质分析  

Differential proteomic analysis in stage Ⅱ breast infiltrating ductal carcinoma tissue with negative estrogen receptor

作　　者：李万锋[1] 张彬[2] 周欣吉林大学分子酶学工程教育部重点实验室 郝东云[3] 范志民[1] 

机构地区：[1]吉林大学第一医院乳腺外科,吉林长春130021 [2]内蒙古民族大学附属医院胸外科,内蒙古通辽028007 [3]吉林大学分子酶学工程教育部重点实验室,吉林长春130124

出　　处：《吉林大学学报（医学版）》2009年第2期360-364,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(200505188)

摘　　要：目的:探索雌激素受体阴性(ER-)Ⅱ期乳腺浸润性导管癌的蛋白标志物,阐述获得蛋白在肿瘤浸润、转移方面的意义。方法:利用二维凝胶电泳(2-DE)、基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF-MS)及在线数据库检索,对5例Ⅱ期乳腺浸润性导管癌ER-癌组织及癌旁组织的差异表达蛋白质进行鉴定分析。结果:5例癌旁正常乳腺组织中检测出的蛋白点数从779到1328不等,实体瘤组织中检测出的蛋白点数从988到1453不等,两者间的匹配点从310到774不等。5例患者乳腺癌组织和癌旁正常乳腺组织的全蛋白差异表达凝胶图谱上确定3个差异蛋白质点S1、S2和S3,鉴定出3个候选蛋白:免疫球蛋白λ链可变区(S1)、胶原蛋白Ⅵ-3链前体(S2)及S100A11钙结合蛋白(S3)。其中S2和S3在5例癌组织中表达均上调(丰度差异2倍以上)。结论:优化的实体瘤组织二维电泳实验条件能够从实体瘤组织中更有效地提取蛋白质;胶原蛋白Ⅵ-3链前体及S100A11钙结合蛋白可能与ER-Ⅱ期乳腺浸润性导管癌的浸润及转移有关。Objective To explore potential biomarker candidate（s） in stage Ⅱ breast infiltrating ductal carcinoma with negative estrogen receptor （ER） and to discuss the significance of the identified proteins in tumor invasion and metastasis. Methods Two-dimensional polyacrylamide gel electrophoresis （2-DE） in combination with matri-assisted laser desorption/ionization tandem time-of-flight mass spectrometry （MALDI-TOF-MS） and bioinformatics analysis were performed to investigate differential proteins of the infiltrating ductal carcinoma at stage 11 and adjacent normal breast tissues from 5 breast cancer patients with negative estrogen receptor. Results The number of the tested protein spots from 5 adjacent normal breast tissues was from 779 to 1328. The number of the tested protein spots from 5 breast cancer tissues was from 988 to 1453. The correspondent spots was from 310 to 774. Three differential proteins （S1, S2, S3） from the 2-DE gel images of 5 patients＇ cancer tissues and adjacent normal tissues were obtained. Three protein candidates were identified： immunoglobulin lambda chain variable region （S1）, collagen alpha-3（Ⅵ） chain precursor（S2） and S100 calcium binding protein All（S3）. Proteins S2 and S3 were found to be up-regulated in all 5 cancer tissues （abundance changed more than 2 folds）. Conclusion Proteins can be obtained efficiently from tissues under the condition of optimized protocols of tissue 2D-PAGE; Collalzen alpha-3（Ⅵ） and S100 calcium binding protein are likely involved in invasion and metastasis in the stage Ⅱ breast infiltrating ductal carcinoma with negative estrogen receptor.

关 键 词：浸润性导管癌 乳腺肿瘤 受体 雌激素 蛋白质组 二维电泳/质谱 

分 类 号：R737.9[医药卫生—肿瘤]