硅藻DNA片段在表达质粒pMZR中再克隆的研究  

The Study on Subcloning Algae DNA Fragment Into Expression Vectors pMZR

作　　者：周金鑫[1] 温晋[1] 

机构地区：[1]暨南大学生物学系

出　　处：《暨南大学学报（自然科学与医学版）》1990年第3期66-70,共5页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：用部分酶切法切开表达质粒 pMZR 中一个 HindⅢ位点后,插入了一个带 HindⅢ粘性末端的1kb 硅藻 DNA 片段重组 DNA 分子转化 E.coli LE392,对转化子中的重组 DNA 分子用酶切分析后,鉴定了1kb DNA 片段插入 pMZR 的位置。Partial digestion was Carried out with the restriction enzyme Hind Ⅲ in the plasmid pMZR containing two Hind Ⅲ sites.The linearized pMZR vectors are recovered from agarose gel slice and treated by BAP to remove the 5' phosphate.After vector preparation,the fragments of 1050 bp ligated together with the prepared vectors.This ligation material is transformed into cell of E.coli LE 392 according to the procedures described by Hanahan. Several transformants are obtained using the antibiotic resistance gene on the plasmid with the plate of SOB medium plus ampcillin.The recombinants DNA extracted from transformants are identified by the analysis of DNA fragmetns after enzymatic cleavage.

关 键 词：表达质粒 部份酶切 DNA重组子 

分 类 号：N55，R[自然科学总论]