胃腺癌的免疫组化及粘液组化研究  

IMMUNOHISTOCHEMICAL AND MUCOHISTOCHEMICAL STUDIES IN GASTRIC ADENOCARCINOMA

作　　者：王克宁[1] 王伟[1] 张北华 孙可为[1] 

机构地区：[1]广州军区武汉总医院病理科

出　　处：《肿瘤防治研究》1989年第4期203-205,258,共4页Cancer Research on Prevention and Treatment

摘　　要：采用免疫金银染色方法,在70例胃腺癌中显示纤维连接蛋白(FN)及层粘连蛋白(LN)的分布。观察表明:部分胃腺癌细胞呈FN和/或LN阳性反应。高、中分化腺癌细胞浆FN阳性率略低于低分化腺癌及粘液腺癌,细胞表面FN阳性率高于胞浆。癌细胞中FN与LN的分布明显相关。肿瘤间质中存在丰富的FN。LN则主要存在于癌巢及血管基底膜上。癌细胞浆中FN阳性率与粘液染色分型明显相关,胃型胃癌最低,肠型胃癌最高。结合临床资料分析,癌细胞FN、LN阳性率与肿瘤浸润、转移,发生部位及肉眼类型无关。Fibronectin (FN) and laminin (LN) in 70 cases with gastric adenocarcinomawere studied histologically by immunogoldsilver staining method. Tumour cells insome specimens showed positive reactions of EN and/or LN. The positive rate ofFN in the cytoplasma of poor differentiated carcinoma was higher than that ofwell differentiated carcinoma. There was no relation between metastasis of carci-noma and positive rate of FN and LN. FN was abundant in tumour stroma, andLN located mainly in the basement membranes of the carcinoma and blood vessels.We believe that there is a correlation between the distribution of FN andLN in tumour cells. With reference to the muco-staining classification, thepositive rate of FN in cytoplasma is higher in gastric types and lower inintestinal types.

关 键 词：胃腺癌细胞 低分化腺癌 肿瘤间质 细胞浆 阳性反应 肿瘤浸润 癌巢 层粘连蛋白 金银染色方法 胃腺瘤 

分 类 号：R73[医药卫生—肿瘤]