DOUBLE POINTS SITE-SPECIFIC MUTATION OF A GENE FOR α-HUMAN CALCITONIN GENE RELATED PEPTIDE  

作　　者：杨迪 闵永洁 施文 陈晖 王启松 

机构地区：[1]Institute of Genetics, Fudan University, Shanghai [2]Shanghai Institute of Biological Products

出　　处：《Chinese Science Bulletin》1989年第20期1758-1758,共1页

摘　　要：Oligonucleotide directed mutagenesis is one of the fundamental methods in the study of protein engineering. The methods previously described have their own drawbacks, for instance, they are time-consuming and can only be used for a single point site-specific mutation.

关 键 词：consuming instance MUTAGENESIS directed MUTATION annealing DNA SAVING POLYMERASE 代军 

分 类 号：N[自然科学总论]