CONSTRUCTION OF Sesbania rostrata COSMID GENOMIC LIBRARY AND MOLECULAR CLONING OF LEGHEMOGLOBIN GENE  

作　　者：刘阳 范云六 

机构地区：[1]Laboratory of Molecular Biology. Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing 100081, PRC

出　　处：《Science China Chemistry》1989年第12期1457-1464,共8页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China.

摘　　要：Using plant mini-Ti cosmid pEND4K, the cosmid genomic library of Sesbania rostratawas constructed. Sizes of plant DNA inserts in clones were 25- 33 kb. The restrictionmapping showed that different recombinant involved various types of plant DNA insert. 4clones containing leghemoglobin gene sequence of S. rostrata were obtained by in situ hy-bridization of colonies. The cloning of leghemoglobin gene sequence has been confirmedby plasmid DNA dot hybridization and Southern blot hybridization.Using plant mini-Ti cosmid pEND4K, the cosmid genomic library of Sesbania rostrata was constructed. Sizes of plant DNA inserts in clones were 25- 33 kb. The restriction mapping showed that different recombinant involved various types of plant DNA insert. 4 clones containing leghemoglobin gene sequence of S. rostrata were obtained by in situ hy- bridization of colonies. The cloning of leghemoglobin gene sequence has been confirmed by plasmid DNA dot hybridization and Southern blot hybridization.

关 键 词：SESBANIA ROSTRATA plant cosmid GENOMIC library LEGHEMOGLOBIN gene molecular cloning 

分 类 号：O6[理学—化学]