卡那霉素链霉菌营养缺陷型菌株的筛选  

SIEVING OF AUXOTROPH OF STREPTOMYCES KANAMYCETICUS

作　　者：宋德贵 黄鹤 

机构地区：[1]广西师大生物系 [2]湖北中医研究院

出　　处：《广西师范大学学报（自然科学版）》1989年第1期59-63,共5页Journal of Guangxi Normal University:Natural Science Edition

摘　　要：通过亚硝基胍(NTG)(在浓度为0.2M,pH为6.0的磷酸缓冲液中)对卡那霉素链霉菌的孢子(浓度为10~6/ml)进行诱变处理,筛选出营养缺陷型的菌株。共检出3株营养缺陷型的菌株,检出率为0.12%.经两代传代培养,性状稳定。然后对其中一株用生长谱的方法进行初步鉴定,为胞嘧啶缺陷型,它的回复突变率小于10^(-7)。The conidiospores of Streptomyces kanamyceticus were mutagenized with the nitrosoguanidine. Then, colonys, each of which is that single mutagenized conidiospore breeds,were sieved for auxotroph of streptomyces kanamyceticus, three auxotroph were got. The rate of mutation is 0.12%. By appraised, one of them is cytosine.

关 键 词：卡那霉素链霉菌 营养缺陷型 突变 孢子 

分 类 号：N55，G658.3[自然科学总论]