天然三倍体鲫鱼突变体萍乡肉红鲫线粒体细胞色素b基因序列分析  

Sequence Analysis of Mitochondrial Cytochrome b Gene from Natural Triploid Mutant,Carassius auratus var.pingxiangnensis

作　　者：付小全[1] 曾柳根[1] 洪一江[1] 盛军庆[1] 王军花[1] 桂建芳[2] 

机构地区：[1]南昌大学生命科学学院,南昌330031 [2]淡水生态与生物技术国家重点实验室,武汉430072

出　　处：《动物学杂志》2009年第2期97-101,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30660143);“973”项目(No.2004CB117401);江西省教育厅项目(2003);淡水生态与生物技术国家重点实验室项目(No.2008FB004)

摘　　要：用细胞色素b(Cyt b)基因特异性引物,对萍乡肉红鲫(Carassius auratus var.pingxiangnensis)的线粒体Cytb基因进行PCR扩增和双向测序。在12个个体中均得到序列一致的Cytb基因全序列,长度为1 140 bp。其A、T、G、C含量分别为28.2%(321)、28.8%(328)、14.8%(170)和28.2%(321),A+T含量(57%)明显高于G+C含量(43%),与其他水生动物相同基因片段碱基含量相似。该基因中密码子第1位核苷酸中4种碱基组成较为均衡;第2位核苷酸中T的使用率较高,为41.8%,G的使用率较低,为13.2%;密码子第3位A的使用率较高,为39.7%,而G的使用率较低,仅为6.3%。BLAST结果显示,萍乡肉红鲫与其他鲫鱼类的Cytb基因具有较高的同源性,其中与银鲫(C.a.gibelio)的相似性为93%,与普通鲫鱼(C.carassius)的相似性为98%。邻接法构建的分子系统进化树表明,萍乡肉红鲫与普通鲫鱼亲缘关系最近。Mitochondrial DNA cytochrome b （Cyt b ） gene was amplified by PCR and sequenced bidirectionally in Pingxiang Red-transparent Crucian Carp （ Carassius auratus var. pingxiangnensis ）, a natural triploid Crucian Carp mutant in the area of Pingxiang City of Jiangxi Province. The results showed that there were no differences in sequences among the twelve samples. The gene sequence was 1 140 bp in full length which contained 28.2% （321）, 28.8% （328）, 14.8%（170）, 28.2%（321） of A, T, G, and C, respectively. The result of A＋T（57%） 〉 G ＋ C （43 % ） was similar to that in other aquatic animals. The first codon evenly contained 4 nucleotides; the second codon had high content of T （41.8 % ） but low content of G （13.2 % ）; the third codon contained 39.7 % of A and 6.3% of G. The Cyt b gene of the Pingxiang Red-transparent Crucian Carp was proved to have high homology with other species of crucian carp recorded in the GenBank. The sequence similarity between this mutant crucian carp and the Silver Crucian Carp （ C. a. gibelio ） was 93 % , and that between this mutant and Common Crueian Carp （ C. carassius ） was 98 % . The result of phylogenetic tree constructed by Neighbor-Joining method indicated the closest phylogenetie relationship between the Pingxiang Red-transparent Crucian Carp and the Common Crueian Carp.

关 键 词：萍乡肉红鲫 线粒体 CYT B基因 序列分析 

分 类 号：S917.4[农业科学—水产科学]