FISH技术在儿童ALL TEL-AML1融合基因检测中的应用价值  

Value of Interphase Fluorescence in Situ Hybridization in Identification of TEL-AML1 Fusion Gene in Pediatric Cases with Acute Lymphoblastic Leukemia

作　　者：王恺[1] 周永安[1] 苏丽萍[1] 解菊芬[1] 赵瑾[1] 马莉[1] 石磊[2] 

机构地区：[1]山西医科大学第二医院血液科 [2]山西医科大学第二医院风湿科,太原030001

出　　处：《中国现代医生》2009年第10期5-6,F0003,共3页China Modern Doctor

摘　　要：目的探讨双色间期荧光原位杂交(I-FISH)技术在儿童急性淋巴细胞白血病(t12;21)形成的TEL-AML1融合基因检测中的应用价值。方法联合双色间期FISH和常规染色体核型分析技术(CCA)对31例儿童初治ALL的骨髓有核细胞进行(t12;21)TEL-AML1融合基因检测。结果7例患儿经FISH检测发现TEL-AML1融合基因,占总病例的22.6%;而CCA检测均未发现有可疑的(t12;21)。结论FISH技术较常规染色体核型分析技术特异性强、敏感度高,可以有效检测出TEL-AML1融合基因,从而为儿童ALL的诊断确立、采用个体化治疗方案提供重要的依据。Objective To discuss the role of interphase fluorescence in situ hybridization（I-FISH） in t（12;21） detection in pediatric cases with acute lymphoblastic leukemia. Methods TEL-AML1 fusion gene was identified in bone marrow mononuclear cells from 31 newly diagnosed childhood ALL patients by dual color I-FISH and conventional cytogenetic analysis（CCA）. Results 7 cases were found with a TEL-AML1 fusion gene by FISH. The incidence of the t（12;21） was 22.6% in newly diagnosed pediatric ALLs. None of those cases had a dubious TEL-AML1 fusion gene when detecting by CCA. Conclusion Dual color I-FISH is more sensitive and characteristic than conventional cytogenetic analysis（CCA） in the identification of TEL-AML1 fnsion gene.So it is palying an significant role in diagnosis and therapy of pediatric cases with acute lymphoblastic leukemia.

关 键 词：急性淋巴细胞白血病 荧光原位杂交 TEL-AML1融合基因 

分 类 号：R733.71[医药卫生—肿瘤]