VEGF-C、VEGFR-3、CD105及CD68蛋白在食管鳞状细胞癌组织中的表达及意义  

Expression of VEGF-C,VEGFR-3,CD105 and CD68 protein in esophageal squamous cell carcinoma and their significance

作　　者：陈明勇[1] 庞霞[1] 郑湘予[1] 李晟磊[1] 赵志华[1] 张红新[1] 陈奎生[1] 

机构地区：[1]郑州大学第一附属医院病理科河南省肿瘤病理重点实验室,河南省郑州市450052

出　　处：《世界华人消化杂志》2009年第6期578-583,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨VEGF-C、VEGFR-3、CD105及CD68蛋白在食管鳞状细胞癌(ESCC)组织中的表达及其与血管和淋巴管转移的关系.方法:应用免疫组化SP法检测50例ESCC、19例癌旁不典型增生组织及50例正常食管黏膜组织中VEGF-C、VEGFR-3、CD105和CD68蛋白的表达.分析这些蛋白表达与ESCC临床生物学行为的关系.结果:ESCC、癌旁不典型增生组织及正常黏膜组织中VEGF-C、VEGFR-3、CD105和CD68蛋白的阳性表达率依次降低(VEGF-C:82.0%,47.4%,26.0%;VEGFR-3:72.0%,36.8%,18.0%;CD105:30.53±7.42,0,0;CD68:50.89±10.36,14.10±3.59,11.30±3.72),组间比较差异均有统计学意义(P<0.05);VEGF-C、CD105和CD68蛋白均与肿瘤的TNM分期、浸润深度、淋巴结转移密切相关(均P<0.05),VEGFR-3蛋白表达与肿瘤的淋巴结转移密切相关(均P<0.05).ESCC组织中VEGF-C蛋白表达与VEGFR-3呈正相关关系(P<0.01),VEGF-C和VEGFR-3阳性表达及阴性表达的病例分别与肿瘤相关的巨噬细胞(tumor-associated macrophages,TAMs)计数和CD105阳性表达血管数组间比较,差异均有统计学意义(P<0.05).结论:联合检测VEGF-C、VEGFR-3、CD105和CD68蛋白可望成为食管鳞癌早期诊断、判断预后及预测淋巴管及血管转移的分子指标之一,为临床的免疫治疗和抗淋巴管及血管转移治疗提供客观、科学、可靠的依据.AIM： To explore the expression of VEGF-C, VEGFR-3, CD105, CD68 protein and their correlation with lymphatic and vascular metastasis in esophageal squamous cell carcinoma （ESCC）.METHODS： Immunohistochemical SP method was used to detect the expression of VEGF-C, VEGFR-3, CD105 and CD68 protein in 50 cases of esophageal squamous cell carcinoma, 19 cases of adjacent atypical hyperplasia epithelium and 50 cases of normal esophageal epithelium. Re-lationship between expression of proteins and clinical biology of ESCC was investigated.RESULTS： The expression rates of VEGF-C, VEGFR-3, CD105 and CD68 protein decreased by turns in carcinoma, adjacent atypical hyperplasia epithelium and normal esophageal epithelium specimens （VEGF-C： 82.0%, 47.4%, 26.0%; VEGFR-3： 72.0%, 36.8%, 18.0%; CD105： 30.53 ＋ 7.42, 0, 0; CD68：50.89 ＋ 10.36, 14.10 ± 3.59, 11.30 ± 3.72）. There was a significant statistic dif- ference among the groups （P 〈 0.05）. The expression of VEGF-C, CD105 and CD68 protein was closely correlated with the TNM grade, infiltrative depth and lymphatic metastasis in esophageal squamous cell carcinoma （all P 〈 0.05）. The expression of VEGFR-3 protein was closely correlated with the lymphatic metastasis in esopha- geal squamous cell carcinoma （all P 〈 0.05）. The expression of VEGF-C protein had positive correlation with the expression of VEGFR-3 （P 〈 0.01）. There were statistically significant differ- ences among positive and negative expression of VEGF-C and VEGFR-3 groups, and TAMs count and positive expression of CD105 blood vessel groups （P 〈 0.05）.CONCLUSION： VEGF-C, VEGFR-3, CD105 and CD68 protein play important roles in the infiltration, vascular metastasis and carcinogenesis in esophageal carcinoma. Joint detection of VEGF-C, VEGFR-3, CD105 and CD68 protein is expected to be one of molecular index in the early diagnosis, prognostic judgment and vas- cular metastasis of esophageal squamous cell carcinoma, offering an objective and reliable parameter for the

关 键 词：食管肿瘤 鳞状细胞癌 血管内皮细胞生长因子-C 血管内皮细胞生长因子受体-3 CDL05 CD68 浸润 转移 免疫组化 

分 类 号：R735.1[医药卫生—肿瘤]