DNA异倍体联合C-12检测在良恶性腹水中的诊断价值  

Diagnostic values of DNA-aneuploidy and C-12 analysis for benign and malignant ascites

作　　者：刘利炜[1] 陈振东[1] 孙昕[2] 李俊[1] 吴秀伟[1] 何远春[1] 李嘉嘉[2] 

机构地区：[1]安徽医科大学第一附属医院肿瘤内科,安徽省合肥市230022 [2]安徽医科大学第一附属医院中心实验室,安徽省合肥市230022

出　　处：《世界华人消化杂志》2009年第6期632-635,共4页World Chinese Journal of Digestology

摘　　要：目的:评价检测腹水细胞的DNA异倍体及肿瘤标志物表达鉴别良恶性腹水的价值.方法:68例有明确诊断的腹水患者,分为良性组31例,恶性组37例.每位患者除常规腹水脱落细胞学检查外,还分别进行FCM-DNA倍体分析和使用C-12多肿瘤标志物蛋白芯片检测肿瘤标志物.结果:良恶性腹水DI、增值指数有显著差别(P<0.01).FCM-DNA倍体检测对恶性腹水的敏感性为72.97%,特异性为93.54%,准确性为82.35%.C-12检测恶性腹水的敏感性为94.59%,特异性为54.84%,准确性为76.47%.两者联合检测敏感性达94.59%,特异性达96.77%,准确性为82.35%.结论:FCM-DNA倍体检测与C-12多肿瘤标志物联合检测可以提高诊断的敏感性和特异性,对良恶性腹水的鉴别诊断具有较大的价值,易于临床应用.AIM： To evaluate the diagnostic values of DNA- aneuploidy and tumor markers for benign and malignant ascites.METHODS： Ascites fluid specimens were collected from 68 patients with ascites（benign 31, malignant 37）. Besides routine exfoliated cytology examination, FCM-DNA ploidy analysis was performed, and tumor markers were detected by C-12 multiple tumor marker protein chip.RESULTS： There were significant differences in DNA index and proliferation index between be- nign and malignant ascites groups. The sensitivity of FCM-DNA and that of C12 for malignant ascities were 72.97% and 94.59%, respectively while specificities were 93.54% and 54.84%. The accuracy of FCM-DNA and that of C12 were 82.35% and 76.47%, respectively. The combined sensitivity, specificity and accuracy were 94.59%, 96.77% and 82.35 %, respectively.CONCLUSION： Joint detection of DNA-aneuploidy and C-12 multiple tumor marker protein chip could improve the sensitivity and specificity of ascites. They might be of high diagnostic values for benign and malignant ascites and might benefit further clinic procedures.

关 键 词：腹水 诊断 流式细胞术 C-12 肿瘤标志 

分 类 号：R572[医药卫生—消化系统] R730.4[医药卫生—内科学]