梗阻性黄疸大鼠NF-κB的变化及其对免疫应答的影响  

Variation of NF-κB and its effect on immune response in rats with obstructive jaundice

作　　者：成小林[1] 时开网[1] 徐锦[1] 李伟[1] 

机构地区：[1]南京医科大学附属南京第一医院普外科,江苏省南京市210006

出　　处：《世界华人消化杂志》2009年第7期662-666,共5页World Chinese Journal of Digestology

基　　金：南京市卫生局基金资助项目;No.200507003~~

摘　　要：目的:探讨梗阻性黄疸(obstructive jaundice,OJ)时NF-κB的变化及其对免疫应答的影响.方法:60只Wistar♂大鼠随机分成3组:假手术组(SHAM组)、梗阻性黄疸组(CBDL组)和梗阻性黄疸+NF-κB抑制剂脯氨酸二硫化氨基甲酸酯(PDTC)组(PDTC组).每组术后7、14d分批(n=10)检测光镜下肝脏病理组织学,血清总胆红素(TB),谷丙转氨酶(ALT),内毒素(LPS)水平,肝组织促炎因子IL-1β、IL-6,抑炎因子IL-10以及NF-κB蛋白表达.结果:CBDL组7、14d大鼠均出现肝组织病理损伤,CBDL组较SHAM组血清TB、ALT、LPS增高(7d:140.14±10.17vs7.309±1.04,134.479±10.20vs35.79±3.76,189.33±11.05vs2.816±0.58;14d:194.608±12.73vs36.142±3.51,217.797±12.37vs7.321±1.03,292.816±14.53vs2.664±0.53,均P<0.01),肝组织IL-1β,IL-6,IL-10和NF-κB表达增强(均P<0.01),且14d较7d变化更为显著.PDTC组大鼠血清TB、ALT和肝组织IL-1β、IL-6、NF-κB表达在7d时相点时比CBDL组显著下降(P<0.01),而到14d时相点时比较CBDL组无明显变化;LPS和IL-10表达与CBDL组各时相点相比无明显差异.结论:梗阻性黄疸大鼠早期(7d)通过PDTC抑制NF-κB活化表达,可下调促炎因子的表达,减轻肝损.后期(14d)作用不明显,其机制可能是通过LPS、IL-10等其他途径所致.AIM： To investigate the variation of NF-KB and its effect on immune response in rats with obstructive jaundice （OJ）.METHODS： Sixty male Wistar rats were randomized into 3 groups, that is, sham operation group, common bile duct ligation （CBDL） group, and CBDL＋PDCT group. Liver histopathology, levels of serum TB, ALT and LPS, liver IL-1β IL-6, IL-10 and NF-kB were detected at 7 d and 14 d after operation respectively in each group （n = 10）.RESULTS： In CBDL group, all rats were injured with the liver histological damage, in which the level of serum TB, ALT, LPS were all increased as compared with those in SHAM group 7 d and 14 d post-operation （7 d： 140.14 ± 10.17 vs 7.309 ± 1.04, 134.479 ± 10.20 vs 35.79 ± 3.76, 189.33 ± 11.05 vs 2.816 ± 0.58; 14 d： 194.608 ± 12.73 vs 36.142 ± 3.51, 217.797 ± 12.37 vs 7.321 ± 1.03, 292.816 ± 14.53 vs 2.664 ± 0.53, all P 〈 0.01）, be- sides, expressions of IL-1β, IL-6, IL-10 and NF- nB in liver were increased at day 7 （P 〈 0.01） and even more significant at day 14 （P 〈 0.01）. After PDTC administration, compared with those in CBDL group, the level of serum TB, ALT as well as the expressions of IL-1β, IL-6 and NF-KB in liver were all significantly decreased at 7 d （P 〈 0.01）, while at 14 d there was no significant dif- ference. However, after PDTC administration, compared with those in CBDL group, there was no significant difference for the level of LPS and IL-10 at both 7 d or 14 d.CONCLUSION： In the early days （7 d）, PDTC does play a protective role in OJ by down- regulating the activation of NF-KB and down- regulating IL-lβ and IL-6, so as to alleviate the injuries of liver tissues. While in the late days （14 d）, PDTC has no significant effect, which may be caused by LPS, IL-10 and other functional channels.

关 键 词：梗阻性黄疽 核因子KB 脯氨酸二硫化氨基甲酸酯 血清总胆红素 谷丙转氨酶 内毒索 

分 类 号：R363[医药卫生—病理学]