乙型肝炎病毒cccDNA与HBx蛋白在肝细胞性肝癌中表达的关系及意义  

Relationship between hepatitis B virus covalently closed circular DNA and HBx protein expression in hepatocellular carcinoma and its significance

作　　者：潘爱萍[1] 黄古叶[2] 陈晶[3] 何燕玲[4] 

机构地区：[1]广西中医学院第一附属医院检验科,广西壮族自治区南宁市530023 [2]广西中医学院第一附属医院肝病科,广西壮族自治区南宁市530023 [3]广西中医学院第一附属医院分子生物学实验室,广西壮族自治区南宁市530023 [4]广西中医学院第一附属医院病理科,广西壮族自治区南宁市530023

出　　处：《世界华人消化杂志》2009年第7期712-715,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨肝细胞性肝癌中乙型肝炎病毒(HBV)cccDNA与HBx蛋白表达的关系及其意义.方法:取42例肝细胞性肝癌患者的癌和癌旁组织,采用SABC法检测组织中的HBx蛋白;采用RT-PCR法检测组织中的HBV cccDNA水平.结果:HBx蛋白在癌及癌旁组织中的阳性例数分别为31例(73.8%)和35例(83.3%),无显著性差异;癌旁组织中cccDNA水平较癌组织中的高,但是统计学检验无显著性差异;癌组织和癌旁组织中HBx蛋白(+)者的cccDNA水平均明显高于HBx蛋白(-)者(P<0.05).HBx蛋白表达与cccDNA水平呈正相关(r=0.778,P<0.01).结论:HBx蛋白的表达与cccDNA水平明显相关,他们相互作用、相互影响并在肝癌的发生发展中起重要作用.AIM： To explore relationship of hepatitis B virus covalently closed circular DNA （HBV cccDNA） expression to the expression of HBx protein within hepatocellular carcinoma and to investigate their significance.METHODS： The tumor tissues and their adja- cent-tumor tissues of 42 HCC cases were selected. The expression of the HBx protein was detected by SABC immunohistochemistry and the expression levels of hepatitis B virus covalently closed circular DNA were measured by real time polymerase chain reaction （RT-PCR）.RESULTS： The expression of the HBx protein was negative in normal liver tissues while there were 31 positive cases （73.8%） in tumor tissues and 35 positive cases （83.3%） in adjacent-tumor tissues without significant difference. The levels of HBV cccDNA in the adjacent-tumor tissues were higher than those in HCC tissues but no significant difference was detected between them. The levels of HBV cccDNA in tumor tissues whose HBx proteins expression were positive were higher than those whose HBx proteins expression were negative （P 〈 0.05）, and similar result existed in the adjacent-tumor tissues （P 〈 0.05）. There was a positive correlation between HBx protein expression and the expression lev- els of HBV cccDNA （r = 0.778, P 〈 0.01）.CONCLUSION： There is a positive correlation between the expression of the HBx protein and the level of HBV cccDNA, and their interaction might have a very important effect on emergence and development of HCC.

关 键 词：共价闭合环状DNA 乙型肝炎病毒X蛋白 肝细胞性肝癌 适时聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]