两种缢蛏线粒体基因全序列扩增方法的比较  

Comparison of Two Methods Involved in Amplification of Sinonovacula constricta Complete Mitochondrial Genomes

作　　者：郑润玲 李家乐[1] 牛东红[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海200090

出　　处：《生物技术通报》2009年第4期119-121,134,共4页Biotechnology Bulletin

基　　金：国家“863”计划项目(2006AA10A410);上海市重点学科建设项目资助(Y1101);福建省海洋与渔业局重点项目(闽海渔2007015)

摘　　要：介绍了应用长PCR技术扩增缢蛏线粒体全序列的两种方法,并进行了比较。一种方法是使用通用引物扩增COI基因和16S基因并测序后,在两基因的两端分别设计两对引物扩增两基因中间的大片段,分别得到了9.7 kb和7.3 kb的扩增产物。另一种方法是在COI基因序列两端设计一对引物,扩增整个线粒体全序列,得到了17.1 kb的扩增产物。两种方法在缢蛏的研究中进行比较后发现,前一种方法在操作实用性和后期测序方面都比后者好。The Razor Clam（Sinonovacula constricta） is one of the most widely cultured and valuable shellfishes. Two methods were introduced and compared to amplify the complete mitochondrial genomes of the Razor Clam using LA-PCR. One method was to amplify and sequence the genes of 16S and COI with universal primers. The two products of amplification were obtained, the length of which were 9.7 kb and 7.3 kb respectively. The other method was to design one pair of primers which amplified the whole mitochondrial genomes. The length of the amplification product was 17.1 kb. The first method was better in the practical operation and the sequence work than the other one.

关 键 词：长PCR 缢蛏 线粒体全序列 

分 类 号：Q78[生物学—分子生物学] S917.4[农业科学—水产科学]