枸杞子脂肪酸成分的GC/MS分析  

Analysis of Fatty Acids Components of Medlar by GC/MS

作　　者：沈宏林[1,2] 向能军 高茜[1,2] 倪朝敏 缪明明 

机构地区：[1]红塔烟草集团有限责任公司技术中心,云南玉溪653100 [2]昆明理工大学化学工程学院,云南昆明650224

出　　处：《质谱学报》2009年第2期99-104,共6页Journal of Chinese Mass Spectrometry Society

摘　　要：以10%硫酸甲醇-正己烷溶液为甲酯化试剂,利用微波辅助直接甲酯化药材,采用气相色谱-质谱(GC/MS)联用技术研究中药枸杞子脂肪酸成分。根据GC/MS标准质谱数据库检索,结合有机质谱学规律,对直链饱和脂肪酸甲酯、单不饱和脂肪酸甲酯和双不饱和脂肪酸甲酯的裂解规律做了分析和归纳,建立了特征离子确定其碳数及双键数目的方法。运用峰面积归一化法定量分析,鉴定出枸杞子脂肪酸21种,占总成分的93.794%。The fatty acids components of medlar were studied by gas chromatography-mass spectrometry, using CH3 OH （10 % H2 SO4 ）-C8 H14 as the methyl esterification solvent, the direct methyl esterification of fatty acids components in the Chinese material under the microwave-assisted conditions. According to GC/MS standard mass spectrometry database index combined with the regular patterns of organic spectrometry, the structure elucidation of the straight chain saturated fatty acids, single unsaturated fatty acids and double unsaturated fatty acids were analyzed. The novel method was established that can ascertain carbon atom number and double bond number in fatty acids using characteristic ions. The fatty acids components are quantified by the accuracy of area percent method. 21 fatty acids components are identified, accounting for 93. 794% of the total components.

关 键 词：气相色谱-质谱 脂肪酸 枸杞子 

分 类 号：O657.63[理学—分析化学]