IL-17A及Th17在炎症性肠病中的作用  

Role of IL-17A and Th17 in inflammatory bowl disease

作　　者：杨淑萍[1] 智绪亭[2] 卢雪峰[1] 冯立娟[1] 王琴伊[1] 

机构地区：[1]山东大学齐鲁医院消化内科,山东济南250012 [2]山东大学齐鲁医院普外科,山东济南250012

出　　处：《中国现代普通外科进展》2009年第3期222-225,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:检测IL-17A、IL-10、IFN-γ在缓解期炎症性肠病(inflammatory bowl disease,IBD)患者中的表达,探讨诸炎症因子及Th17在炎症性肠病的发病及治疗中的作用。方法:应用ELISA检测12例Crohn病(Crohn disease,CD)、46例溃疡性结肠炎患者(ulcerative colitis,UC)和20例健康人(正常对照组)血清中IL-17A、IL-10和IFN-γ的水平,并用免疫组织化学法检测CD、UC及正常对照组结肠黏膜局部IL-17A的表达。结果:正常对照组结肠黏膜局部未检测到IL-17A的表达,而UC和CD组在结肠黏膜活组织中均检测到IL-17A的表达。同样UC和CD组血清中IL-17A明显增高,而正常对照组血清未检出IL-17A;UC和CD组患者血清IL-10水平均较对照组升高(P<0.05),且CD组明显高于UC组(P<0.05);CD、UC组与正常对照组血清中IFN-γ的表达差异无统计学意义(P>0.05)。结论:IL-17A不仅在缓解期IBD患者结肠黏膜局部表达,同时在其血清中高表达,提示Th17及其分泌的IL-17A在缓解期IBD患者的发病过程中起重要作用,并伴随促炎因子和抑炎性因子失调。Objective： To detect the expression of IL-17A,IL-10 and IFN-γ in patients wth different forms of inflammatory bowel disease （IBD） during clinical remission phase and investigate their role in the onset and treatment of IBD. Methods： Tissue samples were obtained from patients with ulcerative colitis （UC, n=46）, Crohn＇s disease （CD, n=12）, and normal colorectal tissues （n=20）. IL-17A expression was evaluated by a standard immunohistochemical procedure. Serum IL-17A IL-10 and IFN-γ levels were determined by ELISA. Results： IL-17A expression was not detected in samples from normal colonic mucosa but was present in the mucosa of IBD. The level of IL-17A significantly increased in IBD patients while it was not detected in the sera of normal individuals. The level of IL-10 in patients with Crohn＇s disease was significantly higher than that in patients with ulcerative colitis and control group （P〈0.05 and P〈0.05, respectively）. Conclusions： The expression of IL-17A in both the mucosa and serum of remission IBD patients increased significantly, the imbalance between production of proinflammatory and anti-inflammatory cytokines persisted even during remission of the disease.

关 键 词：结肠炎 溃疡性 CROHN病 白细胞介素17 白细胞介素10 

分 类 号：R574.62[医药卫生—消化系统]