小白菊内酯对胃癌BGC823细胞凋亡表型的影响  

The effect on apoptotic phaenotype of gastric cancer cell BGC823 induced by Parthenolide

作　　者：赵立杰[1] 李岩[1] 

机构地区：[1]中国医科大学附属盛京医院消化内科,辽宁沈阳110004

出　　处：《现代肿瘤医学》2009年第4期621-624,共4页Journal of Modern Oncology

摘　　要：目的:探讨小白菊内酯对胃癌BGC823凋亡表型影响。方法:MTT法检测BGC823增殖能力的变化,同时在倒置显微镜下观察细胞形态;采用流式细胞仪检测细胞线粒体膜电位的变化;ELISA法检测caspase-3活性;western blot法检测细胞Bcl-2表达。结果:Par以浓度和时间依赖方式抑制胃癌细胞增殖,镜下可见细胞凋亡形态学改变。经Par处理后,随着Par浓度增加,细胞线粒体膜电位逐渐下降,经100μmol/LPar处理6h后,细胞caspase-3活性增加,24h后下降,至48h仍保持在高水平,western blot法检测到细胞Bcl-2表达下调。结论:Par通过下调Bcl-2和上调caspase-3活性来诱导细胞凋亡。Objective ：To investigate the effect on apoptotic phaenotype of gastric cancer cell BGC823 induced by Parthenolide. Methods： Cytotoxicity was analyzed by MTT. Morphological change of BGC823 was observed by light microscope. Loss of mitochondrial membrane potential （△ψm） stained with rhodamine123 was examined by flow eytometry. Caspase -3 activity was assessed by ELISA assay . Expression of Bcl -2 protein was detected by Western blot assay. Results： Parthenolide inhibited the proliferation of BGC823 cells in a time and concentration dependent manner. After exposed to parthenolide for 24h, △ψm decreased in a concentration dependent manner. Light microscopy showed apoptotic morphological changes in BGC823 cells. After 100 μmol/L parthenolide treatment for 6 hours, the activity of caspase - 3 increased, then declined gradually, but Caspase - 3 activity remain increased till 48h. Western blot assay showed that after exposed to parthenolide for various time, the expression of Bcl -2 protein declined gradually. Conclusion： Parthenolide can induce apoptosis of BGC823 cells by decreasing Bcl -2 expression and increasing Caspase -3 activity.

关 键 词：小白菊内酯 胃癌 凋亡 BCL-2 CASPASE-3 

分 类 号：R730.53[医药卫生—肿瘤] R735.2[医药卫生—临床医学]