HGPRT缺陷型小鼠肝癌低淋巴道转移细胞的筛选  

The selection of HGPRT defecient cell line from mouse hepatocarcinoma cells

作　　者：纪延[1] 凌茂英[1] 白洁 王波 

机构地区：[1]大连医科大学基础医学院病理教研室

出　　处：《大连医科大学学报》1998年第1期6-7,共2页Journal of Dalian Medical University

基　　金：国家自然科学基金

摘　　要：自细胞融合技术问世以来，已被广泛用于研究肿瘤细胞的恶性表型的表达与调控。这一技术的关键步骤是建立一个次黄嘌呤鸟嘌呤磷酸核糖转移酶（ＨＧＰＲＴ）缺陷型并保持亲代特征的细胞系，这样才可以用ＨＡＴ培养液选择杂交瘤细胞。本文采用浓度梯度递增法从小鼠肝癌低淋巴道转移细胞ＨＣａ－Ｐ中选出８－氮鸟嘌呤（８－Ａｇ）抗性细胞，该细胞既对ＨＡＴ敏感又保持低淋巴道转移特征。Cell fusion has been extensively used in the phenotypic expresion and regulation of malignant cells.The key step of this technique is to obtain a hypoxanthineguanine phosphoribosyltransferase (HGPRT) defecient cells which maintain the characteristics of the wild type cells as one of the parent cells of fusionWe carried out this work on low lymphatic metastatic mouse hepatocarcinoma cell line This cells were converted into HAT sensitive (8-azaguanine resistence)by growing in successively increasing concentration of 8-azaguanineThese 8-azaguanine resistence cells maintain the HAT sensitivity and low lymphatic metastatic ability(186%)

关 键 词：肝癌 细胞融合 HGPRT 淋巴道转移细胞 

分 类 号：R73－3[医药卫生—肿瘤]