P53基因产物的形态定量分析在大肠癌早期诊断中的意义  

Morphonuclear image analysis of P53 gene product is useful for the early diagnosis of colorectal carcinoma

作　　者：陈玲[1,2] 吕申 王焕华[1,2] 夏养志 

机构地区：[1]大连医科大学基础医学院病理教研室 [2]内蒙古医学院病理教研室

出　　处：《大连医科大学学报》1998年第1期14-15,共2页Journal of Dalian Medical University

摘　　要：利用图像分析技术，对１５例正常肠粘膜、４５例癌旁粘膜、３９例大肠腺瘤及６６例大肠癌进行了Ｐ５３基因产物免疫组化染色结果及七项核参数的测定，并对二者进行线性相关分析。结果表明：正常粘膜、癌旁粘膜、大肠腺瘤及大肠癌Ｐ５３基因产物表达阳性率及免疫反应分值（ｉｍｍｕｎｏｒｅａｃｔｉｖｅｓｃｏｒｅＩＲＳ）分别为：０、４４４％、１０２６％、５７５８％；０、０３５、０７６、１０２６。相关分析表明：Ｐ５３免疫组化染色强度及阳性物含量与其相应的细胞核大小及ＤＮＡ含量呈正相关。该结果提示：Ｐ５３基因突变可能与大肠癌的发生有关。Ｐ５３免疫组化染色结合图像分析技术进行ＤＮＡ含量测定，对大肠癌的早期诊断有重要意义。P53 gene product expression detected immunohistochemically and 7 nuclear parameters of 66 carcinomas,39 adenomas,45 mucosa adjacent to carcinoma and 15 normal mucosa in large intestine were examined with Morphonuclear Image Analysis.P53 gene product was detected in 5758%,1026%,444% and 0 in carcinomas,adenomas,mucosa adjacent to carcinoma and normal mucosa respectively.The content and the intensity of P53 gene product expression correlated with DNA content and the size of nucleus of the correspondent cell.The results indicate that the product of inactivated P53 gene perhaps relate to the occurrence of colorectal carcinoma and the examination of DNA content and P53 gene product expression may be useful for the early diagnosis of colorectal carcinoma.

关 键 词：大肠癌 癌前病变 大肠癌 P53基因产物 免疫组化 

分 类 号：R735.340.4[医药卫生—肿瘤]