人乳头瘤病毒16型E6E7反义RNA抑制宫颈癌细胞恶性表型的作用  

The inhibitory effect of antisense-RNA of papillomavirus type 16 E6E7 genes on malignant phenotypes of cervical carcinoma cell line

作　　者：明利华[1] 周兰萍[1] 商铭 孙亚洲 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所

出　　处：《中华实验和临床病毒学杂志》1998年第1期54-57,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的研究癌基因的特异性反义ＲＮＡ对癌细胞生长繁殖和恶性程度的影响。方法用逆转录病毒载体将人乳头瘤病毒（ＨＰＶ）－１６Ｅ６Ｅ７反义ＲＮＡ导入ＨＰＶ－１６ＤＮＡ阳性的宫颈癌细胞株ＣａＳｋｉ中，观察该细胞在导入反义ＲＮＡ后其表型特征和在裸鼠体内致癌能力的变化。结果ＨＰＶ－１６Ｅ６Ｅ７反义ＲＮＡ能降低宫颈癌细胞ＣａＳｋｉ的生长速率，抑制其在软琼脂上的集落形成能力，并能明显地抑制其在裸鼠体内的致癌能力。Ｗｅｓｔｅｒｎｂｌｏｔ分析发现ＨＰＶ－１６Ｅ６Ｅ７反义ＲＮＡ能使宫颈癌细胞中病毒ＨＰＶ－１６Ｅ６基因的表达水平降低。结论ＨＰＶ－１６Ｅ６Ｅ７反义ＲＮＡ能使宫颈癌细胞ＣａＳｋｉ恶性表型逆转；由其引起的癌细胞中ＨＰＶ－１６癌基因表达水平的降低可能是癌细胞表型逆转的原因之所在；ＨＰＶ－１６癌基因的表达水平对维持癌细胞的恶性表型起着重要作用。In our previous study,the malignant phenotypes of the NIH3T3 cells, transformed with HPV-16 E6E7 genes, could be inhibited by the antisense RNA of HPV-16 E6E7. In this study, the antisense RNA of HPV-16 E6E7 was induced into the cervical carcinoma cells, caski, which contained HPV-16 DNA, The results showed that the cellular growth velocity was down regulated by the antisense RNA of HPV-16 E6E7, the ability of forming cellular colonies in soft agar medium and the tumorigenicity in nude mice of CaSki cells were also inhibited by the antisense cellular colonies in soft agar mediam and the tumorigenicity in nude mice of Caski cells were also inhibited by the antisense RNA. In addition, the decrease of the expression of viral early genes in carcinoma cells was detected with Western blot assay.It denoted that the phenotypes and the carcinogenicity of tumor cells were inhibited by the associated antisense RNA,and this may be one of the pathways inducing tumor cells reversion.

关 键 词：逆转录病毒 人乳头瘤病毒 反义RNA 子宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]