用基因工程表达的抗原早期诊断鼻咽癌  

Early diagnosis of nasopharyngeal carcinoma using recombinant antigens expressed in bacterial

作　　者：万卓越[1,2,3] 皮国华[1,2,3] 孙宁 李志仁[1,2,3] 唐慰萍[1,2,3] 谷淑燕 

机构地区：[1]黑龙江省卫生防疫站 [2]中国预防医学科学院病毒学研究所 [3]广东医学院病理教研室

出　　处：《中华实验和临床病毒学杂志》1998年第1期74-76,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的为了建立鼻咽癌（ＮＰＣ）早期诊断方法。方法以基因工程表达的、经纯化的ＥＢ病毒（Ｅｐｓｔｅｉｎ－Ｂａｒｖｉｒｕｓ，ＥＢＶ）早期抗原（ＥＡ）成分ＥＡ－Ｄ和ＥＡ－Ｒ作为诊断抗原，建立了酶联免疫吸附试验（ＥＬＩＳＡ），检查３０例ＮＰＣ病人及４９例正常人血清中的ＥＡ／ＩｇＡ抗体。结果用ＥＬＩＳＡ检测抗体较用细胞涂片免疫酶方法（ＩＥ）敏感。ＥＬＩＳＡ检测ＮＰＣ病人血清中ＥＡ／ｌｇＡ抗体，阳性率为１００％，ＥＡ／ｌｇＡ抗体效价均≥１∶１００。而用ＩＥ法，平行检测３０例ＮＰＣ病人血清中ＥＡ／ｌｇＡ抗体效价，结果６例为阴性（＜１∶１０），抗体阳性率为７０％。ＥＬＩＳＡ明显地提高了ＮＰＣ的检出率。以ｐ１３８（ＥＡ－Ｒ）和ｐ５４（ＥＡ－Ｄ）分别或混合包被，检测对ＥＢＶ特异的ＥＡ－Ｄ和ＥＡ－Ｒ的抗体。结论表明在ＮＰＣ病人血清中存在对ＥＡ两种抗原的抗体，对ＥＡ－Ｄ的抗体滴度高于对ＥＡ－Ｒ的抗体。因此，以两种抗原混合包被作为诊断抗原建立的ＥＬＩＳＡ方法，为ＮＰＣ的早期诊断提供更敏感、特异和简便的手段。An enzyme-linked immunosorbent assay(ELISA) was developed by using recombinant purified early antigens, EA-D and EA-R to detect lgA antibodies in sera from nasopharyngeal carcinoma(NPC)pateints. 30 sera from NPC pateints and 49 sera from normal persons were detected by the developed ELISA. The serum positive rate and antibody titer were compared with immunoenzymatic method(IE)on slide with Raji cells Smears. ELISA is more sensitive and specific. The serum positive rate of NPC patients was increased from 70% by IE to 100% by ELISA and 77% of the serum antibody titer reached≥1∶200. The results show that the expressed recombinant p138 and p54 antigens are effective for detection of EA/IgA antibody in sera from NPC patients and ELISA will be a sensitive, specific and convenient method for early diagnosis of NPC.

关 键 词：鼻咽肿瘤 病毒学 基因工程抗原 抗原 诊断 

分 类 号：R739.630.4[医药卫生—肿瘤]