对氨基苯磺酸降解菌的分离鉴定及降解特性研究  

Isolation and Identification of a 4-Aminobenzenesulphonate Biodegrading Strain and Its Degradation Characteristics

作　　者：王艳青[1] 张劲松[1] 周集体[1] 王竞[1] 

机构地区：[1]大连理工大学环境与生命学院工业生态与环境工程教育部重点实验室,大连116023

出　　处：《环境科学》2009年第4期1193-1198,共6页Environmental Science

基　　金：辽宁省自然科学基金项目(20032122)

摘　　要：对大连某城市污水处理厂活性污泥进行长期驯化,筛选得到好氧条件下以对氨基苯磺酸(4-aminobenzenesulphonate,4-ABS)为唯一碳源和能源生长的高效降解菌株W1.根据菌株W1的形态特征、生理生化特征及16S rDNA序列分析,初步鉴定为Pannonibacter菌属.通过考察生长条件对降解效果的影响,确定了该菌株降解4-ABS的优化条件为:接种量10%、30℃、pH 7、摇床转速150 r/min,并且在有外加碳源的情况下仍保持较高的4-ABS降解活性.在4-ABS的降解过程中,4-ABS自身含有的氨基和磺酸基会以NH4+和SO42-的形式释放到水体中,但浓度仅为理论释放量的77.6%和91.5%,推测原因是部分释放的NH4+和SO42-被菌株W1作为氮源和硫源利用;菌株W1可以耐受2500 mg/L的4-ABS,且在32 h内实现90%的降解率;4-ABS降解率为94.7%时,可以实现84.4%的TOC去除率,且没有检测到其它芳香化合物生成,表明菌株W1可以实现4-ABS的彻底矿化.A 4-aminobenzenesulphonate-degrading strain W1 was isolated from the activated sludge of Dalian municipal wastewater treatment plant. Strain W1 was able to utilize 4-ABS as sole carbon and energy source under aerobic condition. It was identified as Pannonibacter sp. according to its morphological, physiological and biochemical characteristics and the analysis of its 16S rDNA gene. The optimum conditions for 4-ABS biodegradation in the shaking flasks were 10% inoculum, 30 ℃, pH 7.0 and were rotated at speed 150 r/min, respectively. Strain WI could keep high degrading ability even in the presence of extra carbon source. Release of ammonia and sulfate were around 77.6% and 91.5 % of theoretic concentration according to 4-ABS degradation, for 4-ABS could serve as the nitrogen and sulfur source. Strain W1 showed efficient biodegradability even at 2 500 mg/L, and 90% 4-ABS removal was achieved in 32 h. The 84.4% TOC removal was achieved after 94.7% consumption of 4-ABS, and no aromatic intermediates were detected, referring the complete mineralization of 4-ABS by strain W1.

关 键 词：对氨基苯磺酸 生物降解 Pannonibacter sp.W1 矿化 

分 类 号：X172[环境科学与工程—环境科学]