不同浓度四氯化碳复合法诱导家兔肝纤维化模型的建立  

Establishment of liver fibrosis in rabbit model by injecting carbon tetrachloride intraperitoneally and drinking ethanol water

作　　者：曾燕[1] 赵建农[1] 冷志[2] 马英[2] 

机构地区：[1]重庆医科大学附属第二医院放射科,重庆400010 [2]重庆医科大学附属第二医院病理科,重庆400010

出　　处：《第三军医大学学报》2009年第8期694-696,共3页Journal of Third Military Medical University

摘　　要：目的寻找到适宜做影像学和血清学研究的动物家兔肝纤维化模型建立方法。方法分别用体积比为5%四氯化碳(CCl4)油溶液和纯CCl4,按0.1 ml/kg剂量每周1次腹腔注射,并辅以5%浓度的乙醇作为饮水,诱导家兔肝纤维化形成,并于注射后6、8、10、12周分组处死家兔取其肝脏进行病理学检查。结果家兔的死亡多发生在4周之内,6周之后趋于稳定。5%CCl4浓度组家兔死亡率为60%,纯CCl4组死亡率为25%。结论长期给予CCl4可导致家兔的肝纤维化形成,采用腹腔注射纯CCl,同时辅以5%浓度的乙醇作为饮水的复合法造模,家兔死亡率低,成模率高。Objective To explore an ideal approach to establish rabbit model of hepatic fibrosis suitable for radiological and serological research. Methods Totally 40 rabbit models of hepatic fibrosis was established by intraperitoneally injecting 5% carbon tetrachloride in oil solution or 100% carbon tetrachloride （both groups, 0.1 ml/kg, once a week） , and drinking 5% ethanol water. Another 8 rabbits who drank normal water and received a peritoneal injection at 0.1 ml/kg served as control. The rabbits （5 or 2 per time for model group and control） were killed at 6, 8, 10 and 12 weeks respectively after first injection. Their livers were reseeted for gross and morphological observation with HE and Masson staining. Results Death of the models usually happened within 4 weeks after first injection and became stable after 6 weeks. Mortality of 5% carbon tetrachloride group was 60% , but 25% in 100% carbon tetrachloride group. Macroscopy and microscopy indicated that liver fibrosis was observed in rat models. Conclusion Long-term intraperitoneal injection of carbon tetrachloride results in rabbit hepatic fibrosis. Injection of 100% carbon tetraehloride intraperitoneal, at 0. 1 ml/kg, once a week, combined with and 5% ethanol as drinking water is a suitable approach to establish rabbit model of hepatic fibrosis with low mortality and high success rate.

关 键 词：四氯化碳 动物模型 家兔 肝纤维化 

分 类 号：R-332[医药卫生] R575.2