LIGHT基因和干扰素-γ转染诱导肝癌HepG2细胞凋亡的研究  

Apoptosis of HepG2 cells after transfection with LIGHT gene and interferon-γ

作　　者：李金朋[1] 吴力群[1] 韩冰[1] 卢云[1] 吕振华[2] 刘相萍[2] 杨堃[2] 隋爱华[2] 

机构地区：[1]青岛大学医学院附属医院肝胆外科,266003 [2]青岛大学医学院附属医院中心实验室,266003

出　　处：《中华消化外科杂志》2009年第2期124-126,共3页Chinese Journal of Digestive Surgery

摘　　要：目的探讨脂质体介导LIGHT基因和IFN-γ转染诱导肝癌HepG2细胞的促凋亡作用。方法以脂质体介导LIGHT基因和IFN-γ转染肝癌HepG2细胞，设立转染组（单转、联合转染组）和对照组（未转染组）。分别于转染后12、24、48h检测肝癌HepG2细胞的凋亡率、Bcl-2及Caspase-8的表达量，并采用方差分析。结果（1）细胞凋亡率：单转组转染后12、24、48h为18．8％±3．5％、25．7％±2．8％、36．4％±3．6％；联合转染组为23．8％±2．4％、31．1％±2．1％、42．5％±4．5％；对照组为8．7％±2．1％、9．3％±1．6％、10．9％±1．2％。3组比较差异有统计学意义（F=15．69，53．33，48．28，P〈0．01）。（2）Bcl-2表达量：单转组在转染后12、24、48h为16．4％±5．0％、13．4％±3．5％、8．6％±2．3％；联合转染组为14．7％±3．8％、9．1％±2．0％、4．6％±2．0％；对照组为25．3％±6．3％、19．8％±4．4％、10．1％±3．8％。3组比较差异有统计学意义（F=6．19，12．29，5．81，P〈0．05）。（3）Caspase-8表达量：单转组在转染后12、24、48h为19．3％±2．4％、27．2％±1．9％、33．7％±3．0％；联合转染组为22．7％±2．2％、30．9％±3．1％、38．2％±3．2％；对照组为1．2％±0．8％、1．8％±0．6％、3．2％±1．5％。3组比较差异有统计学意义（F=71．54，112．78，101．61，P〈0．01）。结论LIGHT基因转染肝癌HepG2细胞后通过调节Bcl-2及Caspase-8的表达来发挥促凋亡作用，IFN-1能增强LIGHT基因诱导肝癌HepG2细胞的凋亡。Objective To investigate apoptosis of HepG2 cells after transfection with LIGHT gene and interferon-γ. Methods LIGHT gene and interferon-γ were transfected into HepG2 ceils by liposome mediated method. The HepG2 cells were divided into group A （transfected with LIGHT gene or interferon-γ）, group B （transfected with LIGHT gene and interferon-γ） and group C （non-transfection group）. The apoptosis rate of the HepG2 cells and the expression of Bcl-2 and Caspase-8 were detected 12, 24, 48 hours after transfection. Results （ 1 ） The apoptosis rates of HepG2 cells at hour 12, 24 and 48 after transfection were 18.8% ±3.5%, 25.7%± 2.8% and 36.4% ±3.6% in group A, 23.8% ±2.4%, 31. 1%±2. 1% and 42.5%±4.5% in group B, and 8.7% ±2.1% , 9.3%± 1.6% and 10.9% ± 1.2% in group C. There was a significant difference in apoptosis rate among the 3 groups （ F = 15.69, 53.33,48.28, P 〈0.01 ）. （2） The expression of Bcl-2 in HepG2 cells at hour 12, 24 and 48 after transfection was 16.4%± 5.0% , 13.4% ±3.5% and 8.6% ± 2.3% in group A, 14.7% ±3.8%,9.1%±2.0% and 4.6% ±2.0% in group B, and 25.3% ±6.3%, 19.8% ±4.4% and 10.1%±3.8% in group C. There was a significant difference in the expression of Bcl-2 among the 3 groups （F = 6.19, 12.29, 5.81, P 〈 0.05）. （3） The expression of Caspase-8 at hour 12, 24 and 48 after transfection were 19.3% ±2.4%, 27.2%±1.9% and 33.7% ±3.0% in group A, 22.7% ±2.2%, 30.9% ±3.1% and 38.2% ±3.2% in groupB, and 1.2% ±0.8%, 1.8% ±0.6% and 3.2% ±1.5% in group C. There was a significant difference in the expression of Caspase-8 among the 3 groups （ F =71.54, 112.78, 101.61, P 〈 0.01 ）. Conclusions LIGHT gene can significantly promote cell apoptosis through regulating the expression of Bel-2 and Caspase-8. Interferon-γ enhanced the effect of LIGHT gene on the apoptosis of HepG2 cells.

关 键 词：癌 肝细胞 HEPG2细胞 LIGHT基因 干扰素-Γ 凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R730.3[医药卫生—临床医学]