外源性表皮生长因子对口腔鳞癌细胞系尿激酶型纤溶酶原激活剂及其抑制剂活性的影响  

Epidermal growth factor differently modulate activity of urokinase plasminogen activator and plasminogen ac-tivator inhibitor-1 in oral squamous cell carcinoma and metastasis carcinoma cell lines

作　　者：张津 张宗美 金晓明[2] 周民战 贾金林 李俊青 石东晓 

机构地区：[1]东营市人民医院口腔科,山东东营257091 [2]青岛大学医学院附属医院口腔科 [3]东营市疾病控制中心

出　　处：《临床口腔医学杂志》2009年第4期201-203,共3页Journal of Clinical Stomatology

摘　　要：目的:探讨表皮生长因子(Epidermal growth factor,EGF)和口腔鳞癌侵袭转移的关系。方法:采用发色底物反应法测量不同浓度EGF作用于口腔鳞癌TSCCa细胞系及颈淋巴转移癌GNM细胞系后u-PA和PAI-1的活性,同时用Boyden Chamber观察EGF诱导口腔鳞癌TSCCa和GNM细胞转移作用。结果:不同浓度的EGF(0ng/ml,10ng/ml,20ng/ml,40ng/ml,80ng/ml)作用于两种细胞系后,随着外源性EGF浓度的增加,GNM和TSCCa细胞中u-PA和PAI-1的活性均有增加,与对照组相比差异有显著性(p<0.05);而以TSCCa细胞中u-PA和PAI-1的活性增加较为明显,u-PA和PAI-1活性与外源性EGF有剂量依赖关系,用不同浓度10ng/ml,20ng/ml,40ng/ml,80ng/ml的EGF培养TSCCa和GNM细胞2h,GNM和TSCCa细胞穿过滤膜数均有增加,在相同的浓度内,TSCCa细胞较GNM细胞增加较为明显,两者相比差异有显著性(p<0.05)。结论:EGF可促进口腔鳞癌细胞侵袭转移。Objective： To detect the role of epidermal growth factor （EGF） on the invasiveness and metastasis of human oral squamous cell carcinoma. Method： Using chromogenic substrate assay to determine the activity of u-PA and PAI-1 in oral squamous cell carcinoma cell lines TSCCa and metastasis carcinoma cell lines GNM treated by different levels of EGF. Boyder chamber were used to observe the function of metastasis in oral squamous cell carcinoma cells TSCCa and GNM. Result： The different levels of EGF （0 ng/ml, 10 ng/ml, 20 ng/ml, 40 ng/ml, 80 ng/ml） had increased the activity of u-PA and PAI-lin TSCCa and GNM cell lines, Moreover, the activity of u-PA and PAI-1 had more dependent on EGF. The cells in lower chamber were increased after cultured for 2 hours by different levels of EGF,which were remarked higher in TSCCa cell lines than that of in GNM cell lines （P〈0.05）. Conclusion： EGF may be a factor associated with invasive and metastasis potential of oral squamous cell carcinoma.

关 键 词：表皮生长因子 口腔鳞癌 肿瘤转移 纤溶酶原激活剂 纤溶酶原激活剂抑制剂 

分 类 号：R739.8[医药卫生—肿瘤] Q813.1[医药卫生—临床医学]