球孢白僵菌几丁质酶基因chit1真核表达  

Expression of chitinase gene chit1 from Beauveria bassiana

作　　者：宋金柱[1] 王允[1] 杨谦[1] 

机构地区：[1]哈尔滨工业大学生命科学与工程系,哈尔滨150001

出　　处：《哈尔滨工业大学学报》2009年第3期98-101,共4页Journal of Harbin Institute of Technology

基　　金：国家高技术研究发展计划资助项目(2003AA241140)

摘　　要：采用PCR方法从球孢白僵菌的DNA中克隆出几丁质酶基因chit1,并将该序列构建到酿酒酵母(Saccharomyces cerevisiae)诱导型表达载体pYES2上,转化到酿酒酵母H158菌株中,通过Northern检验确定该基因在酿酒酵母中成功表达.经过酶活检测该转化子在培养48 h达到酶活高峰,达0.47 U/mL.Chitinase gene chitl was cloned by the polymerase chain reaction （PCR） and sequenced from Beauveria bassiana. The chitinase gene ehitl was ligated into the yeast expression vector pYES2. The expression vector plasmid was transformed into Saccharomyces cerevisiae H158. Gene expression took place upon induction with 2% galactose. The measurement of enzyme activity shows that the expression production can be expressed in active forms and secreted to the medium; the enzyme activity approaches the peak of 0. 47 U/mL after being cultured for 48 h.

关 键 词：球孢白僵菌 几丁质酶 酿酒酵母 表达 

分 类 号：Q933[生物学—微生物学]