秸秆两步发酵法制备丁二酸研究  

Preparation of Succinic Acid from Crop Straw via Two-step Fermentation

作　　者：李兴江[1] 陈小举[1] 姜绍通[1] 潘丽军[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2009年第8期72-75,共4页Food Science

基　　金："十一五"国家科技支撑计划项目(2007BAD34B01);安徽省自然科学基金项目(070411024);合肥工业大学校科学研究发展基金项目(073002F)

摘　　要：目的:以秸秆为原料进行生物转化制备有机酸。方法:在秸秆汽爆法预处理的基础上,以绿色木霉为菌种进行秸秆降解发酵,对降解单糖接种放线杆菌进行二次发酵制备丁二酸。结果:第一步绿色木霉发酵时,通气量0.3L/L·min,30℃发酵36h,后将发酵扩增8倍进行55℃酶解24h,五、六碳糖累积浓度达到49.4g/L。第二步产丁二酸放线杆菌发酵时,控制温度37℃、罐内CO2压力0.11MPa、转速250r/min,发酵40h,最终产丁二酸累积浓度为67g/L。结论:秸秆制备丁二酸的两步发酵法工艺具有工业推广价值。Objective： To produce a large scale of succinic acid through the bioconversion of crop straw. Methods： Crop straw was pretreated by steam-explosion method. Then Trichoderma viride was used to degradating crop straw in the first-step fermentation and Actinobaccillus succinogenes was used for producing succinic acid in the second-step fermentation. Results： The yield of pentose and hexose is about 49.4 g/L when the conditions of first-step fermentation were as follows： fermentation at 30 ℃ for 36 h, then enlargement of the broth by 8 times of volume and hydrolysis at 55 ℃ for 24 h. The yield of succinic acid is about 67 g/L when the second-step fermentation condition was as follows： jar pressure of CO2 0.11 MPa, rotation rate 250 r/min, and fermentation at 37 ℃ for 40 h. Conclusions： The two-step fermentation technology is worth extending to industrial production of succinic acid from crop straw.

关 键 词：农作物秸秆 丁二酸 产丁二酸放线杆菌 绿色木霉 两步发酵 

分 类 号：Q93[生物学—微生物学]