RP-HPLC同时测定乐脉颗粒中阿魏酸和丹酚酸B含量  

Simultaneous determination of contents of ferulic acid and salviamolic acid B in Lemai Granules by RP-HPLC

作　　者：陈恒冲[1,2] 马彬峡[3] 吴春高[3] 赵怀清[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016 [2]辽宁省本溪市药品检验所,辽宁本溪117000 [3]贵阳医学院药学院,贵州贵阳550004

出　　处：《中成药》2009年第4期554-557,共4页Chinese Traditional Patent Medicine

摘　　要：目的：建立RP—HPLC法同时测定乐脉颗粒（丹参、川芎、赤芍、红花、等）中阿魏酸和丹酚酸B两种有效成分的含量。方法：采用Diamonsil—C18（200mm×4．6mm，5μm）色谱柱；以乙腈-甲酸-三乙胺．水（18．6：2：1：81）为流动相等度洗脱；流速：1．3mL／min；柱温：35℃；检测波长：320nm。结果：阿魏酸和丹酚酸B浓度分别在0．51～5．10μg／mL（r=0．9999）和35．8—358．0μg/mL（r=0．9998）范围内与峰面积线性关系良好；方法平均回收率（n=9）分别为99．4％（RSD=1．0％），99．3％（RSD=0．53％）。结论：本方法简便、准确，重复性好，可用于同时测定乐脉颗粒中阿魏酸和丹酚酸B的含量。AIM： To develop a RP-HPLC method for simultaneously determining two components, ferulic acid and salviamolic acid B in Lemai Granules （Radix et Rhizoma salviae miltiorrhizae, Rhizoma Chuangxiong, Radix Paeoniae ruba, Flos Carthami, etc）. METHODS： The analysis was carried out on a Diamonsil-C18 （200 mm × 4. 6 mm,5 μm）column. The mobile phase was aeetonitrile-formic acid-triethylamine-water（ 18.6：2 ： 1：81,v/v/v） and the flow rate was 1.3 mL/min. The detection wavelength was set at 320 nm and column temperature was at 35 ℃. RESULTS ： The linear ranges of ferulic acid and salviamolic acid B were within 0.51 - 5.1 μg/mL （ r = 0. 999 9） ,35.8 -358.0 μg/mL （r =0. 999 8）, respectively. The average recoveries （n =9） were 99.4% （RSD = 1.0% ） ,99.3% （RSD =0.53% ） , respectively. CONCLUSION： This method is simple and can be used to determine these two components with satisfactory accuracy and reproducibility.

关 键 词：乐脉颗粒 阿魏酸 丹酚酸B HPLC 

分 类 号：R927.2[医药卫生—药学]