白头翁醇提物对三硝基苯磺酸诱导大鼠结肠炎细胞免疫调节的研究  

Effect of radix pulsatillae alcohol extract on colitis of rats induced by trinitrobenzenesulfonic acid

作　　者：张文远[1] 卢磊[1] 

机构地区：[1]四川省人民医院消化内科,四川成都610072

出　　处：《西部医学》2009年第4期536-538,542,共4页Medical Journal of West China

摘　　要：目的探讨白头翁醇提取物对三硝基苯磺酸(TNBS)诱导大鼠结肠炎的抗炎效果和对细胞免疫的调节。方法SD雄性大鼠28只,随机分成白头翁组(n=10),模型组(n=10),对照组(n=8)。白头翁组(n=10)和模型组建立TNBS大鼠结肠炎模型。白头翁组和模型组大鼠,用含30 mg TNBS的TNBS 50%乙醇溶液0.85 ml灌肠,对照组给予等量生理盐水灌肠。造模后白头翁组大鼠用白头翁醇提物灌肠,每天1次,模型组和对照组以等量生理盐水灌肠。造模后第8天断颈处死大鼠,进行疾病活动指数(DAI)和组织学损伤评分,ELISA法测定结肠组织中肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平。免疫组化学法检测结肠及脾脏组织CD4+T细胞、CD8+T细胞的表达。结果白头翁组DAI和组织学损伤评分较模型组低(P<0.01)。结肠组织TNF-α、IL-6水平白头翁组低于模型组(P<0.05或P<0.01)。白头翁组结肠组织CD4+T细胞表达低于模型组(P<0.05),而CD8+T细胞表达高于模型组(P<0.05)。白头翁组脾脏组织CD8+T细胞表达高于模型组(P<0.05)。结论白头翁醇提物对TNBS诱导大鼠结肠炎有抗炎作用,可能是通过对CD4+T、CD8+T细胞的免疫调节,促使细胞因子和炎症介质水平降低,而达到抗炎效果。Objective To investigate the effect of radix pulsatillae alcohol extract on colitis of rats induced by trini- trobenzenesulfonic acid. Methods 28 male rats were randomly divided into radix pulsatillae alcohol extract treated group （group A, n=10）, TNBS control group （group B, n=10） and normal control group （group C, n= 8）. 0.85 ml enema containing 30 mg TNBS dissolved in 500% ethanol was instilled into the colon of the rats in group A and group B to induce distal colitis. The rats in group A were perfused through colon with radix pulsatillae alcohol extract at the dosage of 2g/ （kg · d） for 1 ml solution for 7 days, whereas the rats in group B and group C were treated with distilled water. The ani- mals were sacrificed at the 8th day of experiment. The disease activity index （DAD and the tissue damages of colonic mucosa were observed. The levels of tumor necrosis factor a （TNf-α） and interleukin-6 （IL-6） in colonic tissue were measured with enzyme-labeled immunosorbent assay （ELISA）. The expression of CD4^T cells and CD8^T cells in colonic tissue and spleen was detected with immunohistochemistry. Results Compared with group B, the disease activity index （DAD and histological damages of colonic mucosa in group A were significantly improved, the levels of TNF-α, IL-6 of colonic tissue and CD4^＋T lymphocytes in colonic tissue in group A were significantly decrease （P〈0.05）, and CD8^＋T lymphocytes in colonic tissue and spleen in group A were significantly increased （P〈0. 05）. Conclusion The radix palstillae alcohol extract demonstrated an anti-inflammatory action on colitis induced by trinitrobenzenesulfonic acid, and its mechanism might be involved in immunoregulating CD4^＋ and CD8^＋ T lymphocytes and reducing the levels of TNF-α and IL-6 in colonic tissue.

关 键 词：白头翁醇提物 TNBS 细胞免疫 CD4+T细胞 CD8+T细胞 肿瘤坏死因子α 

分 类 号：R574.62[医药卫生—消化系统]