NADE与MATH2蛋白相互作用的研究  

Preliminary study on the interaction between human proteins NADE and MATH2

作　　者：温见燕[1] 龚燕华[2] 王程[3] 阴彬[2] 潘琳[4] 于长安[1] 柯元南[1] 

机构地区：[1]中日友好医院全国中西医结合心血管病中心,北京100029 [2]中国医学科学院基础医学研究所北京协和医学院医学分子生物学国家重点实验室,北京10005 [3]北京大学医学部生理与病理生理学系,100191 [4]中日友好医院临床医学研究所,北京100029

出　　处：《中国分子心脏病学杂志》2009年第2期75-78,共4页Molecular Cardiology of China

基　　金：国家自然科学基金资助项目(30700323)

摘　　要：目的探讨NADE与MATH-2蛋白之间相互作用及MATH-2对NADE蛋白功能的调控效应。方法用GST-pulldown与免疫共沉淀(CoIP)和细胞共定位方法验证NADE与MATH-2蛋白之间的相互作用,用通过流式细胞仪检测蛋白相互作用对凋亡的影响。结果NADE与MRTH2蛋白存在相互作用,这两个蛋白能共定位于SH-SY5Y细胞的胞浆中。二者之间的相互作用能够降低UV引起的细胞凋亡。结论研究首次证实了NADE与MATH2之间的相互作用,发现该相互作用可能通过干扰NADE对紫外线诱发的细胞凋亡产生影响。Objective The interaction between human proteins NADE and MATH2 was examined. Methods The human NADE was used as a bait to search for partners in human brain eDNA library by yeast two-hybrid screening. The interaction was confirmed in vitro and in vivo by GST pull-down and co-immuno- precipitation, and subeellular co-localization was carried out in SH-SY5Y cells. Fluorescence activated cell sorting （FCAS） was used to examine the influence of this interaction on UV-induced apoptosis in 293T cells. Results The protein interaction was validated both in vitro and in vivo. Moreover, the subcellular localization assay showed that the two proteins could co-localize in the cytoplasm of SH-SY5Y cells. Co-transfeetion of MATH2 and NADE protects against UV-induced apoptosis in 293T cells. Conclusions A new protein interaction between NADE and MATH2 was identified. The interaction exerts impact on UV-induced apoptosis in 293T cells.

关 键 词：NADE MATH2 相互作用 

分 类 号：R363[医药卫生—病理学]