反相高效液相色谱法测定肿瘤患者血清中的二氢尿嘧啶和尿嘧啶  

The Determination of Dihydrouracil(UH2) and Uracil(U) Concentration in the Serum by Reversed-Phase High Performance Liquid Chromatography

作　　者：孙昕[1] 钱勇[1] 张从军[1] 陈振东[1] 

机构地区：[1]安徽医科大学第一附属医院肿瘤实验室,安徽合肥230022

出　　处：《肿瘤基础与临床》2009年第2期160-162,共3页journal of basic and clinical oncology

摘　　要：目的建立肿瘤患者血清中二氢尿嘧啶和尿嘧啶浓度测定方法。方法应用反相高效液相色谱法，流动相为磷酸盐缓冲溶液（含0.48%三乙胺），流速0.7 mL/min，检测波长206 nm。结果二氢尿嘧啶最低检测限8μg/L，尿嘧啶最低检测限为5μg/L。血清中二氢尿嘧啶和尿嘧啶质量浓度为15.63-500μg/L时，与相应值线性关系良好（r≥0.9959）。血清中二氢尿嘧啶和尿嘧啶回收率分别为74.56%-88.67%和83.95%-69.54%。结论该方法精密度高、简单、快捷。Objective To establish method for determination of dihydrouracil（UH2） and uracil（U） concentration in the serum. Methods The study was carried out on a high performance liquid chromatograph with a reversed-phase ODS column, a mobile phase of phosphate buffer solution（0.48% triethylamine） at 0.7 mL/min, and UV detection at 206 nm. Results The detection limits of UH2 and U were determined to 8 μg/L and 5 μg/L, respectively. Good linearities were observed of UH2 and U in the serum（r≥0. 9959）. The precision of the method was good and the recoveries of UH2 and U were higher than 69.54%. Conclusion The method is rapid, accurate and convenient for determination of UH2 and U concentrations in the serum of the patients.

关 键 词：高效液相色谱法 肿瘤 二氢尿嘧啶 尿嘧啶 

分 类 号：R730.49[医药卫生—肿瘤]