乳腺癌组织中驱动蛋白家族成员1B mRNA水平预测患者预后的临床价值  

Prognostic prediction by detection of KIF1B mRNA level in breast cancer and its clinical significance

作　　者：李林[1] 李晓青[1] 潘秀华[1] 冯玉梅[1] 

机构地区：[1]天津医科大学附属肿瘤医院肿瘤研究所生物化学及分子生物学室乳腺癌防治研究教育部重点实验室,天津300060

出　　处：《中华乳腺病杂志（电子版）》2009年第2期23-27,共5页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：天津市应用基础研究项目(06YFJMJC12900);教育部长江学者和创新团队发展计划(IRT0743)

摘　　要：目的探讨乳腺原发癌组织中驱动蛋白家族成员1B(KIF1B)基因mRNA表达与乳腺癌临床病理因素的关系,评估其对预测患者预后的临床价值。方法采用实时定量RT-PCR方法检测180例乳腺浸润性导管癌原发癌组织中KIF1B mRNA水平;采用受试者工作特征(ROC)曲线确定KIF1B mRNA水平分组的阈值;χ2检验比较组间差异;Kap-lan-Meier法绘制生存曲线;Log-rank时序检验比较组间生存期的差异;多因素Cox回归模型评估KIF1B mRNA水平对患者预后的预测价值。结果KIF1B mRNA水平在各临床病理因素亚组间的差异无统计学意义;KIF1B mRNA低水平组患者5年无病生存率和5年无转移生存率显著低于高水平组(χ2=4.714P=0.030,χ2=4.393P=0.036),而3年无病生存率和3年无转移生存率差异无统计学意义;KIF1B基因表达状态、临床分期、组织学分级和PR状态是患者独立预后因素,KIF1B mRNA低水平组与高水平组患者发生远处转移的风险比为3.53(95%CI为1.54～8.08,P=0.003)。结论KIF1B mRNA在乳腺原发癌组织中下调与患者的预后差相关,且是独立预后预测分子标志物。Objective To clarify the correlation between the mRNA level of 1B （ KIF1 B）, one member of the kinesin family, and clinicopathologic factors in breast cancer, and to evaluate its clinical value in predicling the prognosis of breast cancer patients. Methods Real-time reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the mRNA expression level of KIF1B in primary invasive ductal carcinoma in 180 cases. Receiver Operating Characteristic（ROC） curve was used to identify the cut-off value of KIFIB mRNA level to group the patients. Chi-square test was used to compare the differences between/among groups. Survival analysis was carried out according to Kaplan-Meier analysis. Log-rank test was used to compare the differences of survival between groups. Multivariate Cox proportional hazards regression model was used to eval- uate KIF1B mRNA level for predicting the prognosis of breast cancer patients. Results No significant differ- ences in KIF1B mRNA levels were found between/among groups with different clinicopathologic status. Five- year disease-free survival and metastasis-free survival of patients with low KIFI B were significantly lower than those with high KIFl B （X2 = 4.714, P = 0. 030 ;x2 = 4. 393, P = 0. 036 ） , while three-year disease-free survival and metastasis-free survival between these two groups were not found significantly different. The K1F1 B status, clinical stage, histological grade, PR status were independent prognostic factors of breast cancer by analysis of Multivariate Cox proportional hazards regression model. Patients with low KIF1 B had higher risk of distant metastasis than patients with high KIF1B （ hazard ratio = 3.53, 95% CI = 1.54 - 8.08, P = 0.003 ）. Conclu- sion The under-expressed KIF1B mRNA is correlated with poor prognosis in breast cancer patients, and is an independent molecular marker for predicting prognosis of breast cancer patients.

关 键 词：乳腺肿瘤 驱动蛋白家族成员1B 定量逆转录聚合酶链反应 

分 类 号：R737.9[医药卫生—肿瘤]