毛细管电泳技术在核酸分析中的应用  

Capillary electrophoresis appliying to analyze nucleic acid

作　　者：季颖[1] 王清清[1] 宋海峰[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《国际药学研究杂志》2009年第2期127-130,共4页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(No.30572224;30572197)

摘　　要：毛细管电泳技术以其高效、快速、所需样品少等特点而广泛应用于核酸的分析。本文综述了近5年来毛细管电泳技术在核酸定性、定量和筛选研究中的应用,包括反义寡核苷酸的分析,适配体的筛选,基因突变的分析,此外对微芯片毛细管电泳技术及其在核酸分析研究中的应用进行了介绍,并对其前景进行了展望。Capillary electrophoresis （CE） is widely used in nucleic acid analysis owing to its high efficiency, high speed, and low sample consumption. This article reviews the applications of CE in nucleic acid qualification and quantification analysis in the recent five years, including the analysis of antisense oligonucleotides, the screening of aptamers, and the analysis of gene mutation. Furthermore, a number of applications of microchip CE analysis in nucleic acid are also reviewed. The possible future trends of microchip CE are prospected.

关 键 词：毛细管电泳 反义寡核苷酸 适配体 微芯片毛细管电泳 

分 类 号：Q52-3[生物学—生物化学]