垂体腺苷酸环化酶激活肽对大鼠脑缺血-再灌注后IL-1β表达的影响  

Influence on the expression of PACAP on focal cerebral ischemia-reperfusion injury in rats

作　　者：许培荣[1] 路文革[2] 白宏英[3] 

机构地区：[1]郑州大学医学院实验中心,郑州450052 [2]河南商丘市第一人民医院神经内科,郑州476000 [3]郑州大学第二附属医院神经内科,郑州450014

出　　处：《中国实用神经疾病杂志》2009年第7期21-23,共3页Chinese Journal of Practical Nervous Diseases

摘　　要：目的探讨PACAP对大鼠局灶性脑缺血-再灌注损伤后脑保护作用及保护机制。方法采用大脑中动脉线栓法建立局灶性脑缺血-再灌注模型,对36只大鼠随机分为假手术组、缺血-再灌注组和PACAP组,在大鼠脑缺血2h后进行12h、24h再灌注,比较光镜下细胞损伤变性程度,应用免疫组化法检测IL-1β的表达。结果PACAP组大鼠脑标本光镜下细胞损伤变性程度与缺血-再灌注组相比明显减轻;IL-1β灰度值PACAP组分别为120±5、114±4,与缺血-再灌注组178±4、162±6比较,有显著性差异(P<0.01)。结论PACAP能抑制大鼠局灶性脑缺血-再灌注时IL-1β的表达,可能是其脑保护作用之一。Objective To explore the mechanism of PACAP neuroprotective function on focal cerebral ischemia-reperfusion injury in rats. Methods The model of focal cerebral ischemia-reperfusion injury was made by occluding middle cerebral artery （MCA） for 2h and reperfusing for 12h,24h. Thirty-six male Sprague-Dawley rats were randomized into Sham-operation group, ischemia-reperfusion group and PACAP group. Sections of every rat were processed with HE staining, immunohistochemistry staining. Results Compared to ischemia-reperfusion group, the injury degree of neuron greatly reduced. The section of PAC- AP group showed the number of injuried neuron and inflammatory cells less than that of ischemia-reperfusion group. The expressions of IL-1β in PACAP group were 120±5 and 114±4 . Compared with 178±4 and 162±6 in I/R group, there was significant difference（P 〈0. 01, P 〈0. 01）. Conclusion The administration of PACAP could decrease the expression of IL-1β after cerebral ischemia-reperfusion injury in rats. It is one of the mechanisms of neuroproteetive function.

关 键 词：PACAP 缺血-再灌注 IL-1β大鼠 垂体 

分 类 号：R-332[医药卫生]