胃肠道5-HT内分泌细胞和肥大细胞与肝再生的关系  

Relationship of both 5-HT immunoreactive endocrine cells and mast cells in gastrointestinal tract with liver regeneration

作　　者：张文学[1] 卢正[1] 唐超智[1] 荣换玲[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院 [2]河南省-科技部共建细胞分化调控重点实验室,河南新乡453007

出　　处：《解剖学报》2009年第2期228-234,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30270673);河南省基础与前沿技术研究计划资助项目(82300450550)

摘　　要：目的探讨胃肠道及肝中5-HT免疫活性（5-HT-IR）内分泌细胞和肥大细胞（MC）与肝再生的关系。方法150只SD大鼠随机分为对照组、手术对照（OC）组和实验组。实验组大鼠切除2/3肝（部分肝切除,PH）,分别于术后不同时段取胃、小肠、肝组织。用免疫组织化学技术检测组织中5-HT-IR内分泌细胞和5-HT-IRMC,用甲苯胺蓝（TB）染色技术检测组织中MC。OC组除不切除肝叶外,其他同实验组。结果1.实验组和OC组胃肠中5-HT-IR内分泌细胞数均于PH后2h较对照组下降（胃P〈0.05,肠P〈0.01）,但实验组PH后6～72h极显著多于对照组（P〈0.01）,肝中未见5-HT-IR内分泌细胞。2.除PH后48～96h肝中MC数显著少于对照组（P〈0.05）外,实验组和OC组胃肠及肝中MC数较对照组均无显著差异。3.在胃中5-HT-IRMC数于PH后3～6h和96h显著多于对照组（P〈0.05）,12～72h极显著多于对照组（P〈0.01）;在小肠中PH后2h和120h显著多于对照组（P〈0.05）,3～96h极显著多于对照组（P〈0.01）;肝中PH后48～72h显著少于对照组（P〈0.05）。OC组胃肠和肝中5-HT-IRMC数较对照组均无显著差异;4.MC的5-HT阳性率在胃中于PH后2～96h,在小肠中于PH后0.5～120h逐渐增大,在肝中于1.5～24h有所增加,48～72h明显下降。OC组胃肠和肝中的阳性率较对照组均无明显变化。结论肝切除后的肝细胞增殖期内胃肠道5-HT-IR内分泌细胞和5-HT-IRMC数显著增多,两种细胞可能提供了在肝再生中起重要作用的5-HT。Objective To investigate the relationship between 5-HT immunoreactive （5-HT-IR） endocrine cells and mast cells （MCs） in gastrointestinal tract and liver with liver regeneration. Methods One hundred and fifty adult SD rats were divided into three groups：control,operation control （OC） and test group. After 2/3 rat hepatectomy （partial hepatectomy,PH）,three tissues （stomach,small intestine and liver） at different phases were taken out,then both 5-HT-IR endocrine cells and 5-HT-IR MCs in tissues were detected by immunohistochemical technique and MCs in tissues were identified by toluidine blue technique. In OC group,the protocols were the same,but liver was not resected. Results 1. Compared with the control,the amounts of 5-HT-IR endocrine cells in both stomach and small intestine in test and OC groups decreased 2 hours after PH （stomach P〈0.05,intestinal P〈0.01）,but increased significantly at 6-72 hours （P〈0.01） in test group. 5-HT-IR endocrine cells were not found in liver;2. Concerning the number of MCs in three tissues,there were no differnece between different phases and control except for reducing 48-96 hours after PH in liver in test group; 3.The amounts of 5-HT-IR MCs in stomach increased at 3-6 hours and 96 hours after PH（P〈0.05）,then increased significantly at 12-72 hours （P〈0.01）. The ones in small intestine increased at 2 hours and 120 hours after PH（P〈0.05）,then increased significantly at 3-96 hours （P〈0.01）. The ones in liver was decreased 48-72 hours after PH（P〈0.05）. In OC group,there were no differnece between different phases and control in three tissues. 4. 5-HT positive rate of MCs in stomach increased gradually 2-96 hours after PH,and one in small intestine did 0.5-120 hours （P〈0.05）. 5-HT positive rate in liver were increased slightly at 1.5-24hours,but decreased significantly 48-72 hours after PH. There was no differnece between OC group and control. Conclusion／The amounts of both 5-HT-IR endocrine cells and 5-HT-IR MCs i

关 键 词：肝再生 5-HT 肥大细胞 胃肠道 免疫组织化学 甲苯胺蓝 大鼠 

分 类 号：R364[医药卫生—病理学]