噪声性听力损失易感性与SOD2单核苷酸多态性的关联  

Association between SNPs in SOD2 and noise-induced hearing loss

作　　者：李旭东[1,3] 刘移民[2,3] 刘斌[2] 郭晓[3] 李永胜[3,4] 

机构地区：[1]广东省职业病防治院,广州510030 [2]广州市职业病防治院 [3]中山大学公共卫生学院 [4]东莞市疾病预防控制中心

出　　处：《中华劳动卫生职业病杂志》2009年第4期211-214,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广东省自然科学基金项目（7018635）;广州市医药卫生科技重点项目（2006-ZDi-06）

摘　　要：目的探讨噪声性听力损失（NIHL）易感性与中国汉族人群SOD2rs2842980、rs5746136、rs2758331、rs4880和rs5746092单核苷酸多态性之间的关联。方法利用病例对照研究，通过比较同一噪声接触强度作业人员的左耳3000Hz频段听闯位移情况，筛选出听阈位移最大的10％个体作为易感人群组，共201例，听阈位移最小的10％个体作为耐受人群组，共202例，并进行相关的职业卫生调查和问卷调查。抽取易感人群和耐受人群空腹外周静脉血5ml用于Qiagen试剂盒抽提基因组DNA，TaqMan探针法化学荧光等位基因鉴别试验检测SNP。结果SOD2基因SNPrs4880C等位基凶是NIHL的危险因素，与T等位基因相比，OR值为1．76，95％CI为1．17～2．63，P=0．006。携带CC和CT基因型的个体患NIHL的风险是TT基因型的2．24倍，95％CI为1．43～3．50，校正混杂因素后仍可发现CC和CT基因型是NIHL的保护因素，与TT基因型相比，OR（95％CI）为2．45（1．51—3．96）。结论在中国汉族人群中，噪声性听力损失易感性与SOD2线粒体靶向定位序列的rs4880单核苷酸多态性有关。Objective To observe the association between SNPs in SOD2 and noise-induced hearing loss in Chinese HaM population. Methods A case-control study was designed to study the effect of environmental risk factors on susceptibility to NIHL in 201 sensitive workers and 202 resistant workers. A questionnaire was designed to carry out an investigation, and an occupational health survey was used to identify the occupational risk factors. Genomic DNA was extracted from peripheral blood cells samples using standard procedures of Qiagen kit. SNPs were detected using standard procedures of TaqMan probe allele identification method. Results In SOD2 gene, the C allele of rs4880 was a risk factor of NIHL, compared with the T allele, OR =1.76, 95%CI 1.17- 2.63, P=0.006. CC and CT genotypes compared with Tr were risk factors, crude OR =2.24, 95% CI 1.43- 3.50 and adjusted OR = 2.45, 95% CI 1.51 N3.96, P〈0.001, respectively. Conclusion In Chinese HaM population, SOD2 rs4880 SNP in the mitochondrial targeting sequence is associated with noise induced hearing loss.

关 键 词：噪声 职业性 疾病遗传易感性 多态性 单核苷酸 

分 类 号：R686[医药卫生—骨科学]