外源性FHIT基因对人皮肤癌细胞系A431细胞增殖与凋亡的影响  

Effect of the exogenous fragile histidine triad(FHIT)gene on the proliferation and apoptosis of cutaneous carcinoma cell line A431

作　　者：宋向凤[1] 田中伟[2] 付丹丹[2] 毕新岭[3] 

机构地区：[1]新乡医学院免疫学教研室,上海200433 [2]新乡医学院第一附属医院皮肤科,河南新乡453003 [3]第二军医大学长海医院皮肤科,上海200433

出　　处：《基础医学与临床》2009年第4期405-408,共4页Basic and Clinical Medicine

基　　金：新乡医学院科研启动基金(200612001)

摘　　要：目的研究脆性组氨酸三联体基因(FHIT)对皮肤癌细胞系A431增殖与凋亡作用的影响,探讨FHIT基因在皮肤肿瘤发生发展中的作用。方法脂质体介导的质粒pcDNA3-FHIT转染到有FHIT基因表达异常的皮肤癌细胞系A431,用G418对转染后细胞进行筛选,获得G418抗性的细胞用免疫细胞化学方法进行FHIT基因表达鉴定。用MTT法、集落形成实验及流式细胞仪观察转染前后细胞生长特性的变化。结果转染FHIT基因的A431细胞FH IT蛋白表达阳性,其增殖活性减弱、集落形成能力降低、凋亡率增加,且与对照组相比差异均有统计学意义。结论导入外源性FHIT基因可以抑制皮肤肿瘤细胞A431的增殖,诱导细胞凋亡。Objective To investigate the effect of the exogenous fragile hisdidine triad （FHIT） gene on the proliferation and the apoptosis of cutaneous carcinoma cell line A431, and to explore the mechanism of tumor suppression by the FHIT gene. Methods The plasmids pcDNA3-FHIT and pcDNA3-vector were transfected into the cutaneous carcinoma cell line A431 without FHIT gene expression, and then the transfected cells were screened by G418 and the expression of FHIT was determined by the immunocytochemical staining technique. The effect of FHIT on the growth characteristics of cutaneous carcinoma cell line A431 was observed by MTT, colony forming test and flow cytometry. Results Stable FHIT gene expressing A431 cells were produced, the proliferation activity and colony forming capability of A431-FHIT were suppressed, whereas the apoptosis was increased. All these differences between A431-FHIT cells and the two control groups of cutaneous carcinoma cells had statistical significance. Con- clusion Transfecting the exogenous FHIT gene into cutaneous carcinoma cells line A431can suppress the proliferation of tumor cells, and can also induce apoptosis and cell cycle arrest.

关 键 词：抑癌基因 脆性组氨酸三联体基因 皮肤肿瘤 基因转染 凋亡 

分 类 号：R739.5[医药卫生—肿瘤]