Twist在肺癌中高表达并与肺癌的分化有关  

High Expression of Twist Is Positively Correlated with the Differentiation of Lung Cancer

作　　者：惠林萍[1] 刘冰冰[1] 赵蕾[1] 张四洋[1] 周宝森[2] 邱雪杉[3] 崔泽实[1] 

机构地区：[1]中国医科大学实验技术中心三部,沈阳110001 [2]中国医科大学流行病学教研室 [3]中国医科大学基础医学院病弹学教研室 中国医科大学第一临床学院病理科

出　　处：《中国肺癌杂志》2009年第4期294-299,共6页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金(No.30471493);沈阳市科技局项目(No.2007Gx-20)资助~~

摘　　要：背景与目的转录因子Twist是上皮-间质转变(EMT)过程中的重要调控因子,在肿瘤进展中发挥重要作用。本研究的目的是检测Twist在肺癌组织和细胞系中的表达情况,并探讨其与肺癌的临床病理因素和生物学行为的关系。方法应用免疫组织化学方法检测68例肺癌及8例癌旁肺组织中Twist的表达;采用RT-PCR方法检测人支气管上皮细胞系(HBE)和8个肺癌细胞系Twist1和Twist2mRNA的表达水平;应用免疫荧光方法检测HBE和8个肺癌细胞系中Twist蛋白的表达和亚细胞定位。结果8例癌旁肺组织中Twist弱表达,在68例肺癌组织标本中,9例为Twist弱表达(13.24%),51例为Twist中高表达(75.00%),癌旁肺组织Twist弱表达。Twist高表达与肺癌分化程度(P=0.002)和患者年龄(P=0.012)有关。Twist1和Twist2的mRNA在不同组织学类型的肺癌细胞系中的表达水平均有差异(P<0.05)。免疫荧光显示Twist蛋白在HBE细胞系中弱表达,而在肺鳞癌和腺癌细胞系中高表达,主要定位于细胞浆。结论Twist在正常肺组织中低表达,在肺癌中高表达,其表达水平与肺癌的分化有关,可作为肺癌进展的生物学标志。Background and objective Twist has been identified as a promoting factor for epithelialmesenchymal transition （EMT）, which enhances the metastatic potential of cancer. The aim of this study is to detect the expression of Twist in lung cancer tissues and cell lines, and analyze its relationship with elinieopathologie characteristics and biological behavior of lung cancer. Methods Twist expression was examined in 68 lung cancer specimens and 8 normal lung specimens using immunohistochemistry （S-P method）. Expression levels of Twist1 and Twist2 mRNA were detected using transcription-polymerase chain reaction （RT-PCR） in HBE and 8 lung cancer cell lines. Immunofluorescence was used to detect the Twist protein expression levels and subcellular localization in lung cancer cells and HBE （human normal bronchi epithelium） cells. Results Among 68 lung cancer specimens, 9 samples showed weak expression of Twist 13.24% （9 of 68）, 75.00% （51 of 68） lung cancer specimens showed moderate to strong Twist staining whereas 8 corresponding normal lung specimens showed weak staining extent. Twist expression level was positively correlated with differentiation （P=0.002） and age （P=0.012）. Twistl and Twist2 mRNA expression levels were incompatible in different histology types. The fluorescence signal of Twist protein was conspicuous in lung squamons cell earcinoma cells and adencarcinoma cells, primarily in cytoplasm, but low in HBE. Conclusion High expression of Twist in lung cancer was associated with differentiation. Twist could be used as a valuable biomarker to evaluate the progression of lung cancer.

关 键 词：肺肿瘤 TWIST 肿瘤标志物 

分 类 号：R734.2[医药卫生—肿瘤]