18S rDNA的PCR扩增技术在棘阿米巴角膜炎临床诊断中的应用  

Application of 18S rDNA PCR technique in the laboratory diagnosis of Acanthamoeba keratitis in the clinical

作　　者：朱学军[1] 刘晓燕[1] 苏晓霁[1] 徐国兴[1] 胡健章[1] 

机构地区：[1]福建医科大学附属第一医院眼科,中国福建省福州市350001

出　　处：《国际眼科杂志》2009年第4期715-718,共4页International Eye Science

摘　　要：目的:探讨利用聚合酶链反应(polymerase chain reaction,PCR)技术扩增棘阿米巴18S rDNA在角膜炎早期临床诊断应用中的可行性。方法:以18S rDNA为模板,利用特异性引物JDP1-JDP2配合PCR技术来检测临床标本中的棘阿米巴原虫。结果:在临床拟诊为棘阿米巴角膜炎的16例(16眼)中,通过PCR检测法有12眼证实为棘阿米巴原虫感染,同时100g/L KOH角膜刮片后镜检有5例发现了棘阿米巴包囊。两种诊断方法用Fisher确切概率法统计,P<0.05。结论:18S rDNA PCR技术对正确诊断早期棘阿米巴角膜炎有重要的临床应用价值。AIM：To discuss the feasibility for early diagnosis of Acanthamoeba keratitis using polymerase chain reaction amplification the gene 18S rDNA fragment.METHODS： We used 18S rDNA as template, the A- canthamoeba in clinical speciman is detected by Specific primer JDP1-JDP2 combining PCR technology.RESULTS： In 16 eyes of 16 cases which had been diagnosed Acanthameeba in clinic 12 eyes proved Acan- thamoeba keratitis using PCR, 5 cases found Acantha- moeba follicle by observing the epithelial specimen with 100g/L KOH under microscopy. With the Fisher exact propabillty to add up the two diagnostic method, and P〈 0.05. CONCLUSION： Polymerase chain reaction is of great importance in the correction for early diagnosis of Acan- thamoeba keratitis.

关 键 词：18S RDNA PCR技术 棘阿米巴角膜炎 诊断 

分 类 号：R772.21[医药卫生—眼科] R394[医药卫生—临床医学]