三氯乙烯降解菌FT17的分离、鉴定及其降解特性研究  

Isolation,Identification and Characteristics of a Trichloro-ethylene Degrading Bacterium FT17

作　　者：王新新[1,2] 张颖[1] 李慧[1] 王元芬[1,2] 

机构地区：[1]中国科学院沈阳应用生态研究所陆地生态过程重点实验室,辽宁沈阳110016 [2]中国科学院研究生院,北京100049

出　　处：《微生物学通报》2009年第4期563-568,共6页Microbiology China

基　　金：国家973计划项目(No.2004CB418505);国家自然科学基金项目(No.30670391)

摘　　要：采用水-硅油双相系统,从辽河流域浑河沈阳段底泥中筛选得到一株三氯乙烯降解菌FT17。综合形态特征、生理生化特征、16S rRNA Blast分析和系统发育分析结果,将该菌株鉴定为Sporosarcina ginsengisoli。菌株FT17最适生长温度为34°C,最适生长pH为7.8。苯酚作为共代谢基质可以促进该菌株对三氯乙烯的降解。该菌株的三氯乙烯降解酶在胞内和胞外均存在。采用两种质粒提取方法对该菌株进行质粒检测,结果均没有发现质粒条带,推测该菌株的三氯乙烯降解基因位于染色体上。The bacterium PT17, capable of degrading trichloroethylene, was isolated from trichloroethylene contaminated sediments from the Liaohe River using the water-silicon oil biphasic system. Biochemical, physiological characteristics and phylogenetic study based on the 16S rRNA gene sequences all indicated that strain FT17 should be placed in the Sporosarcina ginsengisoli. The experiment of single factor optimization about the growth of strain FT17 was carried out, and the results showed that the optimum temperature was 34.0℃ and the optimum pH value was 7.8. The degradation ratio of trichloroethylene could be enhanced at the present of 100 mg/L phenol. Intracellular and extracellular degrading enzymes were both important to the degradation of trichloroethylene. Two methods were applied to extract plasmids from strain FT17, yet none of plasmids were detected. So it was presumed that the degrading gene may locate on the chromosomes instead of on the plasmids.

关 键 词：三氯乙烯 生物降解 筛选 鉴定 芽孢八叠球菌 

分 类 号：X172[环境科学与工程—环境科学]