消痰散结方对裸鼠人胃癌MKN-45端粒酶逆转录酶(hTERT)蛋白及mRNA表达的影响  

Influence of Xiaotan Sanjie Fang on Expression of Human Gastric Cancer MKN-45 Telomerase Reverse Transcriptase and mRNA in a Nude Mice Model

作　　者：李春杰[1] 魏品康[2] 

机构地区：[1]中国人民解放军第211医院,黑龙江哈尔滨150001 [2]上海长征医院,上海200003

出　　处：《中华中医药学刊》2009年第1期52-54,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：中国博士后基金资助项目(20060400639)

摘　　要：目的:通过观察消痰散结方对裸鼠人胃癌MKN-45原位移植模型端粒酶逆转录酶(hTERT)蛋白及mRNA表达的影响,探讨其抑制胃癌细胞增殖的作用机制。方法:采用OB胶粘贴法建立裸鼠人胃癌MKN-45原位移植瘤模型,随机分成模型组、消痰散结方低剂量组、消痰散结方中剂量组、消痰散结方高剂量组、5-Fu组,每组8只,运用免疫组化、实时荧光定量RT-PCR方法检测hTERT蛋白及mRNA的表达。结果:①消痰散结方低、中、高剂量组的平均瘤重与模型组相比,有显著差异(P<0.01);②消痰散结方中、高剂量组hTERT蛋白的阳性表达率与模型组相比有统计学意义(P<0.05或P<0.01);③消痰散结方中、高剂量组hTERTmRNA含量与模型组相比有统计学意义(P<0.05)。结论:①消痰散结方有明显的抑制胃癌细胞生长的作用;②消痰散结方通过下调hTERT蛋白及mRNA的表达而抑制端粒酶的活性,使癌细胞端粒长度逐渐缩短,丧失无限增殖能力,起到抑制肿瘤细胞生长、增殖的作用。Objective：To explore the mechanism of Xiaotan Sanjie Fang（ XTF）, a traditional Chinese medicinal empirical formula for curing gastric cancer,in inhibiting proliferation of gastric cancer cells by observing its influence on the expression of human gastric cancer MKN-45 telomerase reverse transcriptase （hTERT） and mRNA in a nude mice in situ tumor transplantation model. Methods：Using OB glue,the nude mice human gastric cancer MKN-45in situ transplantation tumor model was established, based on which 40 animals were equally randomized into 5 groups ： the blank model group, low-dose XTF group, intermediate-dose XTF group, high-dose XTF group, and 5-Fu group, hTERT protein and mRNA expressions were assayed by immunohistoehemistry and RT-PCR. Results ： 1 ） There was a significant difference in mean tumor weight between the low- , intermediate- , and high-dose XTF groups and the model group（P〈0. 01 ） . 2）There was a significant difference in hTERT positive expression between the intermediate- and high-dose XTF groups and the model group （P 〈 0. 05 or P 〈 0. 01 ）. 3 ）There was a significant difference in hTERT mRNA content between the intermediate- and high-dose XTF groups and the model group（ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion ： 1 ） XTF has a marked inhibitory effect on the growth of gastric cancer cells. 2）XTF inhibits hTERT by down-regulating expression of hTERT protein and mRNA, thus shortening the length of telomeres gradually, rendering it unable to proliferate indefinitely and finally inhibiting the grnwth nnd proliferntion of tumor cells.

关 键 词：消痰散结方 胃癌 端粒酶 端粒酶逆转录酶 原位移植 

分 类 号：R-33[医药卫生]